Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

PCR

PCR

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

RAP<sup>2</sup>G: Relation-Aware Progressive Pseudo-label Generation for Cross-subject MI-EEG Recognition.

IEEE transactions on bio-medical engineering·2026

Same author

Comprehensive analyses of carbapenem-resistant and ESBL-producing bacteria in fresh vegetables and their resistome in the United States.

Food research international (Ottawa, Ont.)·2026

Same author

Molecular characterization of Blastocystis sp., Cryptosporidium spp., and Giardia spp. in Eld's deer (Rucervus eldii) and their forest rangers in Hainan, China.

Parasites & vectors·2026

Same author

Multimodal subspace independent vector analysis effectively captures latent relationships between brain structure and function.

Imaging neuroscience (Cambridge, Mass.)·2026

Same author

Synergistic interfacial stabilization and kinetics acceleration in olivine cathodes enabled by rare-earth perovskite engineering.

Chemical communications (Cambridge, England)·2026

Same author

Multi-Echo SSFP: A Method for Synthetic bSSFP MRI Contrast Without Banding Artifacts at High Field.

Magnetic resonance in medicine·2026

Search research articles

Related Experiment Video

Updated: Feb 27, 2026

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Published on: September 3, 2009

Enhanced simultaneous overlap extension-PCR by gold nanoparticles.

Wenchao Yang¹, Xueyan Cao², Xinhui Li³

¹School of Pharmacy, Shanghai University of Medicine & Health Sciences, Shanghai, China.

Nanomedicine : Nanotechnology, Biology, and Medicine

|July 5, 2017

Summary

This study introduces a novel gold nanoparticle-mediated simultaneous overlap extension-PCR (AuNP-mediated SOE-PCR) method for efficiently fusing multiple DNA fragments. This technique simplifies DNA assembly for synthetic biology and protein engineering applications.

Keywords:

DNA fusion DNA mutation Gold Nanoparticles Overlap extension-PCR

More Related Videos

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Published on: October 16, 2018

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Related Experiment Videos

Last Updated: Feb 27, 2026

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Published on: September 3, 2009

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Published on: October 16, 2018

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Area of Science:

Molecular Biology
Synthetic Biology
Biotechnology

Background:

Efficiently fusing multiple DNA fragments is crucial for synthetic biology and protein engineering.
Existing methods can be complex and time-consuming.

Purpose of the Study:

To develop a streamlined method for simultaneous fusion and amplification of multiple DNA fragments.
To enhance the efficiency of overlap extension PCR using gold nanoparticles.

Main Methods:

Development of a gold nanoparticle-mediated simultaneous overlap extension-PCR (AuNP-mediated SOE-PCR) technique.
Optimization of reaction conditions, including increased concentrations of rTaq DNA polymerase and gold nanoparticles.
Testing the method's efficacy with up to six lambda DNA fragments.

Main Results:

The AuNP-mediated SOE-PCR method enables simultaneous fusion and amplification of multiple DNA fragments in a single reaction.
Increased concentrations of rTaq DNA polymerase and gold nanoparticles significantly improve SOE-PCR performance.
Successfully fused up to six lambda DNA fragments using this novel method.

Conclusions:

AuNP-mediated SOE-PCR offers a powerful and efficient approach for DNA fragment assembly.
This method simplifies complex molecular biology workflows in synthetic biology and protein engineering.
The technique is particularly effective for fusing larger numbers of DNA fragments.