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Related Experiment Video

Updated: Feb 27, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

Fluorescence lifetime imaging ophthalmoscopy.

Chantal Dysli¹, Sebastian Wolf¹, Mikhail Y Berezin²

¹Department of Ophthalmology and Department of Clinical Research, Inselspital, Bern University Hospital, University of Bern, Switzerland.

Progress in Retinal and Eye Research

|July 5, 2017

Summary

This summary is machine-generated.

Fluorescence lifetime imaging ophthalmoscopy (FLIO) offers a novel, noninvasive method to analyze retinal diseases by measuring the fluorescence lifetime of endogenous fluorophores. This technique enhances understanding of macular and retinal conditions, aiding in diagnosis and management.

Keywords:

FLIO Fluorescence lifetimes Fluorophore Fundus autofluorescence Metabolism Retinal imaging

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Area of Science:

Ophthalmology
Biophotonics
Medical Imaging

Background:

Retinal autofluorescence imaging is sensitive and noninvasive, primarily measuring lipofuscin fluorescence intensity.
Fundus autofluorescence analysis can be extended beyond intensity to include fluorescence lifetime, reflecting the time fluorophores spend in an excited state.
Fluorescence lifetime imaging ophthalmoscopy (FLIO) enables in vivo measurement of endogenous retinal fluorophore lifetimes.

Purpose of the Study:

To provide a comprehensive overview of fluorescence lifetime imaging (FLI) principles and its emerging role in ophthalmology.
To correlate in vitro measurements of retinal metabolites with in vivo FLIO data.
To review the clinical applications and characteristic FLIO findings in various retinal diseases.

Main Methods:

Explanation of the fundamental concepts and technical aspects of fluorescence lifetime imaging.
Correlation of FLIO data with in vitro measurements of retinal metabolites.
Review of clinical studies detailing FLIO applications in specific retinal pathologies.

Main Results:

FLIO provides unique insights into retinal pathophysiology beyond conventional autofluorescence intensity.
Distinct fluorescence lifetime signatures are observed in diseases like Stargardt disease, AMD, and diabetic retinopathy.
FLIO demonstrates potential for diagnosing and monitoring a range of macular and retinal disorders.

Conclusions:

Fluorescence lifetime imaging ophthalmoscopy is a promising advanced imaging modality for retinal disease assessment.
FLIO expands the diagnostic capabilities of autofluorescence imaging in ophthalmology.
Further research into FLIO applications may reveal new diagnostic and therapeutic strategies for retinal conditions.