Related Experiment Video
Updated: Feb 27, 2026

Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature
Published on: June 19, 2019
Cortisol and Alpha-amylase changes during an Ultra-Running Event
Whitney P Deneen1, Alexis B Jones2
1Department of Natural Sciences, Northeastern State University, Broken Arrow, OK, USA.
Ultra-running significantly impacts athlete stress hormones. Cortisol (CORT) and salivary alpha-amylase (AA) production are suppressed beyond marathon distances, potentially aiding performance and showing sex-based differences.
Area of Science:
- Exercise Physiology
- Neuroendocrinology
- Sports Science
Background:
- Stress hormones like cortisol (CORT) and salivary alpha-amylase (AA) influence athletic performance.
- The impact of extreme endurance exercise, beyond marathon distance, on these hormonal responses is not fully understood.
Purpose of the Study:
- To investigate the effects of ultra-running (distances > 26.2 miles) on the hypothalamic-pituitary-adrenocortical (HPA) axis and autonomic nervous system.
- To compare the production of cortisol (CORT) and salivary alpha-amylase (AA) during long-duration cardio exercise.
Main Methods:
- Saliva samples were collected from participants in an ultra-marathon event (up to 100K).
- Analyzed the neuroendocrine responses, specifically cortisol (CORT) and salivary alpha-amylase (AA) levels.
Main Results:
- Cortisol (CORT) and salivary alpha-amylase (AA) production were suppressed in athletes at distances beyond the marathon.
- The autonomic nervous system's response may be independent of the HPA axis during prolonged physical stress.
- Observed distinct differences in cortisol (CORT) and salivary alpha-amylase (AA) production between male and female athletes.
Conclusions:
- Ultra-running may lead to suppressed cortisol (CORT) and salivary alpha-amylase (AA) levels, potentially benefiting sustained athletic performance.
- The neuroendocrine system exhibits complex responses to extreme endurance exercise, with potential sex-specific variations.
Related Concept Videos
Hypothalamic-Pituitary Axis
Hormones of the Adrenal Glands
The adrenal cortex, a powerhouse of hormone synthesis, generates over two dozen corticosteroid hormones. The zona glomerulosa produces mineralocorticoids, exemplified by aldosterone, influencing the electrolyte composition of body fluids. The synthesis of glucocorticoids such as cortisol and...
Physiological Foundation of Stress
Role of the Sympathetic Nervous System
Adrenaline triggers the...
Hormones Regulating Blood Glucose
In addition to accelerating glucose uptake and utilization, insulin has...
Sympathetic Activation
Exercise Stress Test
Exercise stress testing, commonly known as a treadmill test, is a noninvasive procedure used to evaluate cardiovascular function and diagnose heart conditions.
Definition
An exercise stress test measures the heart's response to exertion using a treadmill or stationary bicycle. Chest electrodes record the heart's electrical activity through an ECG, and blood pressure is monitored regularly.
Purposes

