Receptor mapping in neuropharmacology by autoradiography: some technical problems

In the past decade, substantial advances in receptor mapping have been made. It is now as feasible to carry out receptor localizations as it is to carry out neurotransmitter localizations. A great deal of data has accumulated on various technical procedures and it is clear that significant problems occur with tritiated ligands. These problems involve different autoradiographic efficiencies in tissue regions, and steps must be taken to either correct for these different efficiencies or to eliminate them. It seems that I125-labeled ligands will become more and more important in the field.