Bioactive L acidissima protein hydrolysates using Box-Behnken design.

Area of Science:

• Food Science
• Biochemistry
• Protein Chemistry

Background:

• Limonia acidissima seeds are an underutilized source of valuable proteins.
• Protein hydrolysates possess significant bioactive properties.
• Optimization of extraction and hydrolysis is crucial for maximizing yield and functionality.

Purpose of the Study:

• To optimize the extraction and hydrolysis of proteins from Limonia acidissima seeds.
• To evaluate the antioxidant and metal-chelating activities of the resulting protein hydrolysates.
• To explore the potential application of Limonia acidissima seed protein hydrolysates.

Main Methods:

• Single-factor experiments were conducted to determine optimal extraction parameters.
• Box-Behnken Design (BBD) was employed to optimize the hydrolysis process.
• DPPH radical scavenging, ABTS radical scavenging, reducing power, and metal chelating assays were performed.

Main Results:

• Optimized extraction yielded 924.31 mg/g of total protein under specific conditions (1% alkali, 40°C, 60 min, 1:20 solid to alkali ratio).
• Maximum degree of hydrolysis reached 39.82% using BBD with optimized hydrolysis parameters (pH 2, 2.5% enzyme to substrate ratio, 42.41 min).
• Protein hydrolysates exhibited significant antioxidant activities (32.94% DPPH, 88.18% ABTS) and metal chelating activity (87.39%).

Conclusions:

• Limonia acidissima seeds are a viable source for high-quality protein extraction.
• Optimized hydrolysis significantly enhances the functional properties of seed proteins.
• The protein hydrolysates demonstrate potential for application in functional foods and protein supplements.