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Related Experiment Video

Updated: Feb 27, 2026

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers
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High-Density Serum/Plasma Reverse Phase Protein Arrays.

Cecilia Hellström1, Tea Dodig-Crnković1, Mun-Gwan Hong1

  • 1Affinity Proteomics, School of Biotechnology, SciLifeLab, KTH-Royal Institute of Technology, Box 1031, SE-17121, Stockholm, Sweden.

Methods in Molecular Biology (Clifton, N.J.)
|July 5, 2017
PubMed
Summary
This summary is machine-generated.

High-density protein microarrays enable high-throughput analysis of human serum and plasma. This method allows for the profiling of abundant proteins, aiding in the discovery of diagnostic and prognostic biomarkers.

Keywords:
Affinity proteomicsFluorescent detectionNoncontact inkjet printerPlasmaProtein profilingRPPAReverse phase protein arraySerum

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Area of Science:

  • Biochemistry
  • Proteomics
  • Biomarker Discovery

Background:

  • Human serum and plasma proteomes are rich sources for identifying prognostic and diagnostic biomarkers.
  • High-throughput analysis methods are crucial for increasing statistical power in biomarker studies.
  • Affinity-based microarrays offer a platform for large-scale sample analysis.

Purpose of the Study:

  • To describe a protocol for high-density serum and plasma reverse phase protein arrays (RPPAs).
  • To demonstrate the feasibility of analyzing a large biobank on a single microarray slide.
  • To enable high-quality profiling of abundant target proteins across numerous samples.

Main Methods:

  • Development of a protocol for high-density serum and plasma RPPAs.
  • Immobilization of a biobank containing 12,392 samples onto a single microarray slide.
  • High-throughput assay for profiling abundant target proteins.

Main Results:

  • Successful immobilization and analysis of 12,392 samples on one microarray slide.
  • Demonstration of high-quality profiling of abundant proteins.
  • Validation of RPPAs for large-scale proteomic analysis.

Conclusions:

  • High-density RPPAs provide an efficient method for proteomic profiling of large sample cohorts.
  • This approach facilitates the identification of potential biomarkers from serum and plasma.
  • The described protocol enhances the statistical power of biomarker discovery studies.