Evaluation of key parameters on simultaneous sulfate reduction and sulfide oxidation in an autotrophic biocathode

Bioelectrochemical systems (BESs) are being studied as an alternative technology for the treatment of several kinds of wastewaters with a lack of electron donor such as high-strength sulfate wastewaters. This study evaluates different parameters that influence the simultaneous sulfate reduction and sulfide oxidation in an autotrophic biocathode: ion-exchange membrane (IEM), cathodic pH and cathode potential. Two different membranes were studied to evaluate sulfate and sulfide adsorption and diffusion from the cathode to the anode, observing that a cation-exchange membrane (CEM) widely decreased these effects. Three different cathode pH (5.5, 7 and 8.5) were studied in a long-term operation observing that pH = 7 was the optimal for sulfate removal, achieving reduction rates around 150 mg S-SO₄^2- L^-1 d^-1. Microbial community analysis of the cathode biofilm demonstrated a high abundance of sulfate-reducing bacteria (SRB, 67% at pH 7, 60% at pH 8.5 and 42% at pH 5.5), mainly Desulfovibrio sp. at pH 5.5 and 7 and Desulfonatronum sp. at pH 8.5. The cathode potential also was studied from -0.7 to -1.2 V vs. SHE achieving sulfate removal rates higher than 700 mg S-SO₄^2- L^-1 d^-1 at cathode potentials from -1.0 to -1.2 V vs. SHE. Also, the highest cathodic recovery and the highest sulfur species imbalance were observed at a cathode potential of -1.0 V vs. SHE, which indicated a higher elemental sulfur production.