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Updated: Feb 27, 2026
Extraction of Structural Extracellular Polymeric Substances from Aerobic Granular Sludge
Published on: September 26, 2016
Sources for isolation of extracellular polymeric substances (EPSs) producing bacterial strains which are capable of
Viet-Hoang Nguyen1, Hong-Khanh Nguyen1, Thanh-Dong Nguyen1
1a Institute of Environmental Technology, Vietnam Academy of Science and Technology , Hanoi , Vietnam.
Abstract:
Isolation of extracellular polymeric substances (EPSs) producing bacterial strains capable of using sludge as low-cost growth substrate was carried out in this study. A total of 110 EPS-producing strains were isolated from different sources, which include sludge of beer and winery wastewater treatment plant (WWTP); young, 2-month-old and 10-year-old leachate. Thirty-seven isolated strains showed good growth in sludge medium with cell count varying from 106 to 1010 most probable number (MPN)/mL and total EPS concentration from 2737 to 6639 mg/L. Twenty-one strains produced EPS with high flocculation activity (FAmax varied from 72.0% to 80.2%). The highest FAmax (80.2%) was observed with EPS produced by strain BES 19, which was isolated from sludge of beer WWTP. Sludge of beer WWTP, young leachate and 10-year-old leachate were good sources for isolation of EPS-producing bacteria.
