Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Riboswitches01:56

Riboswitches

9.9K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
9.9K
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

930
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
930
Types of RNA01:23

Types of RNA

73.3K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
73.3K
Types of RNA01:20

Types of RNA

10.1K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
10.1K
Translational Regulation01:29

Translational Regulation

716
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
716
Ribozymes02:47

Ribozymes

13.5K
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...
13.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proximity measurements for objective assessments of crew cohesion in an antarctic space analog environment.

Frontiers in psychology·2026
Same author

Visual Attention and Linguistic Preferences in Time-Telling: A Cross-Linguistic Study of German, Czech, and Russian Speakers.

Journal of psycholinguistic research·2026
Same author

Smartphone Keystroke Biomarkers as Predictors of Adverse Neuropsychiatric Sequelae After Trauma in Trauma Survivors: Prospective Observational Cohort Study.

Journal of medical Internet research·2026
Same author

Clinical Outcomes of 1.5-Stage Arthroplasty for Native Joint Septic Arthritis of the Hip and Knee: A Retrospective Cohort Study.

Arthroplasty today·2026
Same author

Flow Chemistry: Moving beyond Traditional Paradigms.

JACS Au·2026
Same author

Peritraumatic C-reactive protein levels predict pain outcomes following traumatic stress exposure in a sex-dependent manner.

The journal of pain·2026
Same journal

Peripheral B-cell receptor repertoire predicts immune-related adverse events following immune checkpoint inhibitor therapy in advanced renal cell carcinoma.

Scientific reports·2026
Same journal

Effects of black soldier fly (Hermetia illucens L.) larvae zoocompost on the mineral element content of blue honeysuckle berries.

Scientific reports·2026
Same journal

Investigation on absorption refrigeration performance of R1243zf with imidazolium ionic liquid as the working pairs.

Scientific reports·2026
Same journal

DeepTriage-CN: integrating clinical text with vital signs for emergency department admission prediction in an aging population.

Scientific reports·2026
Same journal

Gold nanoparticles as dual-action antiviral agents: disruption of SARS-CoV-2 viral envelopes and RNA integrity.

Scientific reports·2026
Same journal

Comparison of capillary microsampling and venous blood for multi-pathogen serosurveillance.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Feb 27, 2026

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

15.5K

Ribo-attenuators: novel elements for reliable and modular riboswitch engineering.

Thomas Folliard1, Barbara Mertins2, Harrison Steel3

  • 1Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3PJ, UK. thomas.folliard@oriel.ox.ac.uk.

Scientific Reports
|July 6, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a new genetic element, the ribo-attenuator, to improve riboswitches for synthetic biology. This innovation enhances predictability and reliability in gene expression control.

More Related Videos

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.9K
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.5K

Related Experiment Videos

Last Updated: Feb 27, 2026

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

15.5K
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.9K
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.5K

Area of Science:

  • Molecular Biology
  • Synthetic Biology
  • Genetic Engineering

Background:

  • Riboswitches are genetic elements that control gene expression based on small molecule binding.
  • Despite their potential in synthetic biology and bio-manufacturing, riboswitches face limitations like context sensitivity and poor tunability.
  • These limitations hinder their use as modular components in genetic circuits.

Purpose of the Study:

  • To design and introduce a novel genetic element, the ribo-attenuator, to overcome riboswitch limitations.
  • To enable predictable tuning and insulation from genetic context for riboswitches.
  • To enhance the reliability and modularity of riboswitches for biotechnological applications.

Main Methods:

  • Design and implementation of the ribo-attenuator genetic element in bacterial systems.
  • Evaluation of ribo-attenuator performance in terms of tuning predictability and context independence.
  • Comparative analysis of ribo-attenuator function against traditional riboswitches.

Main Results:

  • The novel ribo-attenuator provides predictable tuning of gene expression.
  • Ribo-attenuators insulate riboswitches from variations in genetic context.
  • Expression variation is significantly reduced when using ribo-attenuators.

Conclusions:

  • Ribo-attenuators enhance the modularity and reliability of riboswitches.
  • This innovation advances the application of riboswitches in synthetic biology and bio-manufacturing.
  • Ribo-attenuators represent a significant improvement for engineering gene expression control systems.