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An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic

Enrique Sosa1, Rachel Kim1, Ernesto J Rojas1

  • 1Department of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA.

Stem Cell Research
|July 6, 2017
PubMed

Insights

A new rhesus macaque induced pluripotent stem cell (riPSC) line, riPSC90, was successfully generated and characterized. This riPSC line exhibits key stem cell markers and pluripotency, making it valuable for regenerative medicine research.

Area of Science:

  • Stem Cell Biology
  • Primate Research
  • Regenerative Medicine

Background:

  • Induced pluripotent stem cells (iPSCs) hold promise for regenerative medicine.
  • Establishing reliable primate iPSC lines is crucial for translational research.
  • Previous work has established other rhesus macaque iPSC lines.

Purpose of the Study:

  • To generate and characterize a novel induced pluripotent stem cell line from rhesus macaque embryonic fibroblasts.
  • To authenticate the stem cell properties and pluripotency of the new line.
  • To provide a resource for parallel studies with existing primate iPSC lines.

Main Methods:

  • Generation of iPSCs from rhesus embryonic fibroblast (REF90) cells.
  • Authentication of cell lines using short tandem repeat (STR) analysis.
  • Karyotyping to confirm normal chromosomal complement (42, XY).
  • Analysis of pluripotency markers (OCT4, NANOG, TRA-1-81, SSEA4).
  • In vivo assessment of pluripotency via teratoma formation in immunocompromised mice.

Main Results:

  • Successful generation of the riPSC90 line from REF90 cells.
  • Confirmed genetic identity and normal male karyotype (42, XY) for riPSC90.
  • Demonstrated expression of key pluripotency and self-renewal markers (OCT4, NANOG, TRA-1-81, SSEA4).
  • Confirmed pluripotency through teratoma formation post-transplantation.
  • riPSC90 is suitable for parallel use with previously established riPSC89.

Conclusions:

  • The riPSC90 line is a well-characterized, pluripotent stem cell resource derived from rhesus macaques.
  • This new line expands the available tools for non-human primate stem cell research.
  • riPSC90 offers potential applications in disease modeling and therapeutic development.

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