Related Experiment Video

Updated: Feb 27, 2026

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.7K

MicroRNA-492 overexpression exerts suppressive effects on the progression of osteosarcoma by targeting PAK7

Xuanhe Song1, Yaoping Xie2, Yang Liu1

  • 1Department of Orthopaedic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.

International Journal of Molecular Medicine
|July 6, 2017
PubMed

Insights

MicroRNA-492 (miR-492) acts as a tumor suppressor in osteosarcoma (OS). Lower miR-492 levels correlate with OS progression, and restoring miR-492 inhibits cancer cell growth and invasion.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are key regulators of biological processes.
  • miRNA dysregulation is linked to various cancers, including osteosarcoma (OS).
  • The specific role of miR-492 in OS has not been fully elucidated.

Purpose of the Study:

  • To investigate the function of miR-492 in osteosarcoma.
  • To determine if miR-492 acts as a tumor suppressor or oncogene in OS.
  • To identify potential targets of miR-492 in OS.

Main Methods:

  • Analysis of miR-492 expression levels in OS tissues and cell lines.
  • Overexpression of miR-492 in OS cell lines to assess effects on proliferation, migration, and invasion.
  • In vivo studies using xenograft tumor models.
  • Identification and validation of miR-492 targets, including p21-activated kinase 7 (PAK7).

Main Results:

  • miR-492 expression was significantly downregulated in OS tissues and cell lines.
  • Ectopic miR-492 overexpression suppressed OS cell proliferation, migration, and invasion.
  • miR-492 overexpression attenuated tumor growth in vivo.
  • PAK7 was identified as a direct target of miR-492, with an inverse correlation observed in OS specimens.

Conclusions:

  • miR-492 functions as a tumor suppressor in osteosarcoma.
  • Downregulation of miR-492 contributes to OS progression.
  • miR-492 may serve as a potential therapeutic target for osteosarcoma treatment.

More Related Videos

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

9.9K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.4K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.9K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.8K