Related Experiment Video
Updated: Feb 27, 2026
In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
Published on: April 18, 2025
TGF-β1 promotes human hepatic carcinoma HepG2 cells invasion by upregulating autophagy
1Department of Hepatobiliary Surgery, No. 1 People's Hospital in Jining, Shandong Jining, China. qiaosen12345@126.com.
Objective:
To study the role of TGF-β1 in autophagy and invasion ability of human hepatic carcinoma HepG2 cells.
Materials And Methods:
Cultured HepG2 cells were treated with different concentrations of TGF-β1 for 24 h. The protein expression levels of autophagy relative marker LC3 and Beclin1 were detected by Western blot. The effect of TGF-β1 on invasion ability of HepG2 cells was detected with transwell method.
Results:
The results demonstrated that TGF-β1 was able to activate autophagy of HepG2 cells in a dose-dependent manner. Autophagy inhibitor 3-methyladenine (3-MA) could reverse TGF-β1 induced autophagy process. Also, TGF-β1 significantly promotes the invasion ability of HepG2 cells; however, this process could effectively reverse by autophagy inhibitor 3-MA.
Conclusions:
TGF-β1 enhances HepG2 cells invasion by upregulating autophagy.
Related Concept Videos
01:16TGF - β Signaling Pathway
03:03mTOR Signaling and Cancer Progression
The mTOR pathway or the...