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Related Experiment Video

Updated: Feb 27, 2026

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae
07:40

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae

Published on: December 8, 2017

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A Simple and Inexpensive Microinjection System for Zebrafish Embryos and Larvae.

Seyed-Mohammadreza Samaee1, Hanieh Nikkhah1, Zoltan M Varga2

  • 11 Aquatic Laboratory, Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University , Urmia, Iran .

Zebrafish
|July 6, 2017
PubMed
Summary

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This study introduces a low-cost microinjection system for zebrafish embryos, making advanced research accessible. The new method ensures accurate substance delivery and successful embryo development without significant side effects.

Area of Science:

  • Developmental Biology
  • Biotechnology
  • Zebrafish Models

Background:

  • Microinjection is crucial for in vivo physiological studies.
  • High-cost equipment limits accessibility for many research labs.
  • Zebrafish embryos are a common model organism for microinjection.

Purpose of the Study:

  • To develop an affordable and user-friendly microinjection setup.
  • To validate a simple and accurate calibration method for low-cost systems.
  • To demonstrate the efficacy of the system for zebrafish embryo microinjection.

Main Methods:

  • Assembly of a simple, low-cost microinjection apparatus.
  • Development and testing of a straightforward calibration technique.
  • Verification of injection accuracy and biological impact in zebrafish embryos.
Keywords:
embryo-larvamicroinjection setupzebrafish

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Related Experiment Videos

Last Updated: Feb 27, 2026

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae
07:40

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae

Published on: December 8, 2017

10.4K
Microinjection of Zebrafish Embryos to Analyze Gene Function
07:18

Microinjection of Zebrafish Embryos to Analyze Gene Function

Published on: March 9, 2009

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Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
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Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.

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Main Results:

  • The developed system is inexpensive, easy to assemble, and simple to operate.
  • The calibration method is accurate when compared to high-cost systems.
  • Successful microinjections were confirmed by dye presence, normal development, and larval viability in zebrafish.

Conclusions:

  • This low-cost microinjection system democratizes advanced research techniques.
  • The validated calibration method ensures reliable and accurate substance delivery.
  • The system is effective for physiological studies in zebrafish embryos, promoting wider research participation.