Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Computed Tomography01:10

Computed Tomography

9.1K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
9.1K
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

490
DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
490

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Testicular Photohyperthermia Mediated by Magnetic Nanoparticles: Implications for Male Fertility Control.

Molecules (Basel, Switzerland)·2026
Same author

Impact of oxidative stress on female reproductive parameters: an analysis of systemic and follicular biomarkers.

JBRA assisted reproduction·2025
Same author

Evaluation of Different Cryoprotectant Combinations in Vitrification and Slow Freezing for Ovarian Tissue Preservation in Domestic Cats.

Reproduction in domestic animals = Zuchthygiene·2025
Same author

Tumor-Infiltration Mimicking Model of Contaminated Ovarian Tissue as an Innovative Platform for Advanced Cancer Research.

The AAPS journal·2024
Same author

Decellularized extracellular matrix from bovine ovarian tissue maintains the protein composition of the native matrisome.

Journal of proteomics·2024
Same author

Direct and Abscopal Antitumor Responses Elicited by AlPcNE-Mediated Photodynamic Therapy in a Murine Melanoma Model.

Pharmaceutics·2024

Related Experiment Video

Updated: Feb 27, 2026

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse
12:36

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Published on: September 3, 2021

5.5K

Evaluation of ovarian structures using computerized microtomography.

Fernanda Paulini1, Sacha B Chaves2, José Luiz J P Rôlo1

  • 1Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Asa Norte, 70910-900 Brasília, DF, Brazil.

Anais Da Academia Brasileira De Ciencias
|July 6, 2017
PubMed
Summary

This study explores the use of high-resolution 3D X-ray imaging to examine the internal anatomy of ovaries across seven different mammal species. By comparing these images with traditional tissue analysis, researchers demonstrate that this technology can effectively identify key ovarian features like follicles and blood vessels. This work highlights the potential for non-invasive imaging in future reproductive research and clinical diagnostics.

Keywords:
reproductive biologydiagnostic imagingmammalian anatomyX-ray microscopy

Frequently Asked Questions

More Related Videos

Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary
12:38

Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary

Published on: December 10, 2017

9.1K
A Coregistered Ultrasound and Photoacoustic Imaging Protocol for the Transvaginal Imaging of Ovarian Lesions
10:21

A Coregistered Ultrasound and Photoacoustic Imaging Protocol for the Transvaginal Imaging of Ovarian Lesions

Published on: March 3, 2023

2.4K

Related Experiment Videos

Last Updated: Feb 27, 2026

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse
12:36

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Published on: September 3, 2021

5.5K
Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary
12:38

Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary

Published on: December 10, 2017

9.1K
A Coregistered Ultrasound and Photoacoustic Imaging Protocol for the Transvaginal Imaging of Ovarian Lesions
10:21

A Coregistered Ultrasound and Photoacoustic Imaging Protocol for the Transvaginal Imaging of Ovarian Lesions

Published on: March 3, 2023

2.4K

Area of Science:

  • Reproductive biology and computerized microtomography imaging
  • Veterinary medicine and diagnostic imaging techniques

Background:

Detailed anatomical knowledge of reproductive organs remains a significant challenge for veterinary diagnostics and research. Prior research has shown that traditional histological sectioning provides high resolution but destroys the physical integrity of the specimen. That uncertainty drove the need for non-destructive imaging modalities capable of preserving spatial relationships. No prior work had resolved the full utility of high-resolution X-ray imaging across diverse mammalian species. This gap motivated an investigation into the feasibility of visualizing complex ovarian architecture without invasive tissue processing. Existing imaging techniques often lack the necessary contrast to distinguish fine internal structures within the ovary. Researchers required a reliable method to identify follicles and vascular networks in a three-dimensional context. This study addresses these limitations by applying advanced scanning technology to characterize ovarian morphology across multiple species.

Purpose Of The Study:

The primary aim of this research is to evaluate the utility of computerized microtomography for characterizing ovarian anatomy across multiple mammalian species. Investigators sought to determine if this imaging modality could provide sufficient resolution to identify key internal features. The study addresses the need for non-destructive methods to study reproductive organs without the limitations of traditional tissue sectioning. By exploring diverse species, the team intended to establish a broad baseline for anatomical visualization. This work aims to support advancements in reproductive technologies by providing a clearer understanding of ovarian morphology. The researchers were motivated by the potential to improve diagnostic capabilities for various pathologies. They also aimed to test the feasibility of translating these findings into non-invasive approaches for live subjects. This study provides a necessary assessment of how modern imaging tools can enhance current reproductive biology research.

Main Methods:

The research team conducted a comparative analysis of ovarian specimens collected from seven distinct mammalian species. Each sample underwent high-resolution scanning to generate detailed three-dimensional representations of internal anatomy. Following the digital acquisition, the investigators processed the identical specimens for standard microscopic tissue examination. This dual-modality strategy allowed for the direct correlation between the scan data and established anatomical benchmarks. The review approach focused on identifying specific markers such as the corpus luteum and vascular patterns. Researchers systematically evaluated the clarity of these features across all species to determine the efficacy of the scanning protocol. The study design prioritized the preservation of specimen integrity to allow for subsequent validation. This methodology ensured that the findings regarding anatomical resolution were grounded in verified biological observations.

Main Results:

The scanning process successfully distinguished the cortex and medulla regions in all examined mammalian species. Researchers identified the morphology and distribution of blood vessels with high clarity throughout the ovarian tissue. The imaging clearly observed the corpus luteum and various antral follicles within the specimens. In several instances, the technology allowed for the visualization of oocytes located inside the antral follicles. This study represents the first comprehensive report comparing these specific structures across a wide range of domestic mammals. The results demonstrate that the scanning technique provides a reliable alternative to traditional tissue analysis methods. The data indicate that the approach possesses significant potential for evaluating complex reproductive anatomy. These findings confirm the capability of the technology to capture intricate details of ovarian architecture.

Conclusions:

The authors propose that high-resolution scanning provides a robust platform for characterizing complex ovarian anatomy across diverse mammalian species. This imaging modality successfully identifies internal features such as the corpus luteum and antral follicles. The researchers suggest that the ability to visualize oocytes within follicles represents a significant advancement for reproductive studies. These findings indicate that the technology serves as a reliable alternative to traditional destructive tissue analysis. The team posits that this approach offers a pathway toward non-invasive longitudinal monitoring of ovarian development in living subjects. They emphasize that the technique holds particular promise for small animal models in clinical research settings. The study highlights the potential for future diagnostic applications in identifying various ovarian pathologies. This work establishes a foundation for integrating advanced imaging into standard reproductive biology protocols.

The researchers utilized high-resolution X-ray scanning to identify internal ovarian features. This method allowed for the clear differentiation of the cortex and medulla, alongside the visualization of blood vessels, the corpus luteum, and antral follicles, which were validated against traditional histological tissue analysis.

The study employed computerized microtomography as the primary imaging tool. This technology was selected for its ability to generate three-dimensional reconstructions, which the authors compared against standard histological sections to confirm the accuracy of the observed anatomical structures across the seven mammalian species tested.

The authors note that histological evaluation was necessary as a validation criterion. This step ensured that the structures identified via the scanning process, such as antral follicles and vascular networks, were accurately interpreted when compared to the gold-standard physical tissue sections.

Histological data served as the comparative benchmark to verify the accuracy of the scan results. By preparing the same samples for both imaging and tissue staining, the researchers confirmed that the anatomical features observed in the digital reconstructions matched the physical reality of the ovarian tissue.

The researchers observed that the technique could distinguish between the cortex and medulla regions. Furthermore, they successfully identified the morphology and distribution of blood vessels, as well as the presence of oocytes within certain antral follicles, across the seven different species included in the analysis.

The authors propose that this imaging method could eventually facilitate non-invasive studies in live animals. They suggest this development is particularly attractive for scientific research focused on the progression of ovarian pathologies or the developmental stages of reproductive structures in small animal models.