Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interplay between cohesin and TORC1 links chromosome segregation and gene expression to environmental changes.

eLife·2026
Same author

Disease-specific epigenetic deregulation of enhancers, transposons, and polycomb targets in acute promyelocytic leukemia.

Genome medicine·2025
Same author

Epigenome Mapping in Quiescent Cells Reveals a Key Role for H3K4me3 in Regulation of RNA Polymerase II Activity.

Epigenomes·2024
Same author

The fork protection complex promotes parental histone recycling and epigenetic memory.

Cell·2024
Same author

The exon-junction complex helicase eIF4A3 holds therapeutic potential in acute myeloid leukemia.

Leukemia·2023
Same author

LEO1 Is Required for Efficient Entry into Quiescence, Control of H3K9 Methylation and Gene Expression in Human Fibroblasts.

Biomolecules·2023

Related Experiment Video

Updated: Feb 27, 2026

Genetic Screen for Identification of Multicopy Suppressors in Schizosaccharomyces pombe
13:22

Genetic Screen for Identification of Multicopy Suppressors in Schizosaccharomyces pombe

Published on: September 13, 2022

2.3K

Selecting Schizosaccharomyces pombe Diploids.

Karl Ekwall1, Geneviève Thon2

  • 1Department of Biosciences and Nutrition, Karolinska Institute, Stockholm SE-141 83, Sweden; karl.ekwall@ki.se.

Cold Spring Harbor Protocols
|July 7, 2017
PubMed
Summary
This summary is machine-generated.

This study details methods for selecting diploid yeast strains of Schizosaccharomyces pombe using specific genetic markers. These techniques ensure accurate genetic complementation tests and stable diploid selection for research applications.

More Related Videos

Dissection of Saccharomyces Cerevisiae Asci
12:57

Dissection of Saccharomyces Cerevisiae Asci

Published on: May 19, 2009

19.5K
Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae
05:39

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae

Published on: December 2, 2022

3.3K

Related Experiment Videos

Last Updated: Feb 27, 2026

Genetic Screen for Identification of Multicopy Suppressors in Schizosaccharomyces pombe
13:22

Genetic Screen for Identification of Multicopy Suppressors in Schizosaccharomyces pombe

Published on: September 13, 2022

2.3K
Dissection of Saccharomyces Cerevisiae Asci
12:57

Dissection of Saccharomyces Cerevisiae Asci

Published on: May 19, 2009

19.5K
Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae
05:39

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae

Published on: December 2, 2022

3.3K

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Diploid selection in Schizosaccharomyces pombe is crucial for genetic studies.
  • Heteroallelic complementation using ade6-M210/ade6-M216 is a common method for identifying Ade+ diploids.
  • Sporulation of diploids can be induced by nitrogen starvation, but stable diploids are sometimes required.

Purpose of the Study:

  • To describe reliable procedures for selecting diploid Schizosaccharomyces pombe strains.
  • To provide methods for obtaining stable diploids for genetic complementation tests.
  • To offer alternative auxotrophic markers for diploid selection when ade6 mutations interfere with the gene of interest.

Main Methods:

  • Utilizing ade6-M210/ade6-M216 heteroallelic complementation to select for Ade+ diploids.
  • Employing nitrogen starvation to induce sporulation in selected diploids.
  • Mating h and h mating type strains to prevent sporulation and ensure diploid stability.
  • Using alternative auxotrophic markers for diploid selection when necessary.

Main Results:

  • Established procedures for efficient selection of Ade+ diploids in Schizosaccharomyces pombe.
  • Demonstrated methods for controlling diploid sporulation through mating type selection.
  • Validated the use of alternative auxotrophic markers for diploid selection in specific genetic contexts.

Conclusions:

  • The described procedures facilitate accurate genetic complementation analysis in Schizosaccharomyces pombe.
  • Stable diploid strains can be reliably generated for various research applications.
  • Flexible selection strategies accommodate diverse genetic research needs in yeast.