Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

General Transcription Factors01:30

General Transcription Factors

7.3K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
7.3K
Transcription Factors02:16

Transcription Factors

83.1K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
83.1K
Diversity in Cell Signaling Responses01:22

Diversity in Cell Signaling Responses

8.0K
The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity. 
Graded and Abrupt Responses
Some signaling systems generate...
8.0K
Global Regulatory Systems01:28

Global Regulatory Systems

783
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
783
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

1.7K
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
1.7K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.7K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The subpleural pulmonary microvasculature in newborn yak (Bos grunniens).

Veterinary research communications·2008
Same author

Experimental confirmation of potential swept source optical coherence tomography performance limitations.

Applied optics·2008
Same author

A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice.

Plant physiology·2008
Same author

[Spatial and temporal changes of palatal cell proliferation and cell apoptosis of retinoic acid induced mouse cleft palate in different embryonic stages].

Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology·2008
Same author

Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties.

Developmental and comparative immunology·2008
Same author

Non-Gaussian statistics and superdiffusion in a driven-dissipative dusty plasma.

Physical review. E, Statistical, nonlinear, and soft matter physics·2008

Related Experiment Video

Updated: Feb 27, 2026

High Throughput SiRNA Screening for Chloropicrin and Hydrogen Fluoride-Induced Cornea Epithelial Cell Injury
14:20

High Throughput SiRNA Screening for Chloropicrin and Hydrogen Fluoride-Induced Cornea Epithelial Cell Injury

Published on: June 16, 2018

6.8K

Fluoride resistance capacity in mammalian cells involves complex global gene expression changes.

Shujun Ran1, Ning Sun2, Yun Liu3

  • 1Department of Endodontics and Operative Dentistry Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai Key Laboratory of Stomatology Shanghai China.

FEBS Open Bio
|July 7, 2017
PubMed
Summary
This summary is machine-generated.

Mammalian cells can develop resistance to high fluoride levels through significant gene expression changes. These adaptations involve pathways for stress response and fluoride expulsion, offering insights into fluorosis resistance mechanisms.

Keywords:
fibroblastsfluoridefluoride resistancetranscriptome

More Related Videos

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
08:12

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants

Published on: March 29, 2018

10.8K
Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

4.6K

Related Experiment Videos

Last Updated: Feb 27, 2026

High Throughput SiRNA Screening for Chloropicrin and Hydrogen Fluoride-Induced Cornea Epithelial Cell Injury
14:20

High Throughput SiRNA Screening for Chloropicrin and Hydrogen Fluoride-Induced Cornea Epithelial Cell Injury

Published on: June 16, 2018

6.8K
Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
08:12

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants

Published on: March 29, 2018

10.8K
Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

4.6K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Toxicology

Background:

  • Fluorine is prevalent in the environment and oral hygiene products.
  • Excessive fluoride intake can lead to dental and skeletal fluorosis.
  • Individual resistance to fluoride varies, with underlying mechanisms unclear.

Purpose of the Study:

  • To investigate the cellular mechanisms of fluoride resistance in mammalian cells.
  • To identify genetic pathways involved in adaptation to or expulsion of fluoride.

Main Methods:

  • Development of fluoride-resistant mouse L-929 cell lines.
  • High-throughput RNA-sequencing to analyze global gene expression changes.

Main Results:

  • Fluoride-resistant cells exhibited massive alterations in gene expression compared to wild-type cells.
  • Key biological processes affected include extracellular matrix, stress response, receptor binding, and signal transduction.
  • Findings suggest cellular adaptation and fluoride expulsion mechanisms are activated.

Conclusions:

  • High fluoride doses induce cellular stress and activate adaptive pathways in L-929 cells.
  • Identified pathways are crucial for understanding fluoride adaptation and expulsion.
  • Data may help identify cellular targets for managing fluoride toxicity and fluorosis.