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Cholinergic Receptors: Muscarinic

Cholinergic Receptors: Muscarinic

The pharmacological actions of acetylcholine are elicited via its binding to two families of cholinergic receptors or cholinoceptors, namely, muscarinic and nicotinic receptors. Muscarinic receptors are G protein-coupled receptors and have five subtypes, M1–M5. All mAChR subtypes are activated by acetylcholine and blocked by the antagonist, atropine.
The subtypes M1, M3, and M5 couple with the Gq subunit and activate the phospholipase C (PLC) activity, mobilizing intracellular Ca2+....

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The Use of Cystometry in Small Rodents: A Study of Bladder Chemosensation

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Changes of Bladder M_1,3 Muscarinic Receptor Expression in Rats Fed with Short-Term/Long-Term High-Fat Diets.

Chian-Shiung Lin¹, Tony T-L Wu^2,3, Chin-Hong Chang^4,5

¹Department of Urology, Chi Mei Medical Center-Liouyin, Tainan, Taiwan.

Lower Urinary Tract Symptoms

|July 7, 2017

Summary

High-fat diets cause obesity and reduce bladder M1 and M3 muscarinic receptor expression and function in rats. Long-term high-fat diet feeding leads to bladder muscle dysfunction.

Keywords:

bladder high-fat diet muscarinic receptor obesity polyphagia

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Area of Science:

Physiology
Pharmacology
Metabolic Research

Background:

Obesity is linked to various health complications.
Muscarinic receptors play a crucial role in bladder function.
Dietary habits can significantly impact physiological processes.

Purpose of the Study:

To examine the impact of a high-fat diet (HFD) on rat bladder M1 and M3 muscarinic receptor expression.
To assess the effect of HFD on the contractile function of rat bladder.
To determine the duration-dependent effects of HFD on bladder physiology.

Main Methods:

Rats were fed a high-fat diet (HFD) or normal chow for 8 weeks (short-term) and 24 weeks (long-term).
Body weight, food intake, and blood biochemistry were monitored.
Bladder contractile responses and M1/M3 muscarinic receptor protein expression were analyzed.

Main Results:

HFD induced obesity, polyphagia, and altered blood lipid and glucose levels.
Bladder contractile responses to acetylcholine and bethanechol were significantly reduced in HFD groups.
M1 and M3 muscarinic receptor protein expression progressively decreased with longer HFD exposure.

Conclusions:

High-fat diet consumption leads to obesity and polyphagia in rats.
Both short-term and long-term HFD feeding decrease rat bladder M1 and M3 receptor expression and contractile function.
Prolonged HFD intake results in bladder muscle dysfunction.