Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato

To evaluate the possible photoprotection mechanisms of cyclic and linear electron flux (CEF and LEF) under specific high temperature and high light (HH) stress.

Six-leaf-stage tomato seedlings ("Liaoyuanduoli", n=160) were divided into four parts: Part 1, served as control under 25 °C, 500 µmol/(m²·s); Part 2, spayed with distilled water (H₂O) under 35 °C, 1000 µmol/(m²·s) (HH); Part 3, spayed with 100 µmol/L diuron (DCMU, CEF inhibitor) under HH; Part 4, spayed with 60 µmol/L methyl viologen (MV, LEF inhibitor) under HH. Energy conversion, photosystem I (PSI), and PSII activity, and trans-thylakoid membrane proton motive force were monitored during the treatment of 5 d and of the recovering 10 d.

HH decreased photochemical reaction dissipation (P) and the maximal photochemical efficiency of PSII (F_v/F_m), and increased the excitation energy distribution coefficient of PSII (β); DCMU and MV aggravated the partition imbalance of the excitation energy (γ) and the photoinhibition degree. With prolonged DCMU treatment time, electron transport rate and quantum efficiency of PSI (ETR_I and Y_I) significantly decreased whereas acceptor and donor side limitation of PSI (Y_NA and Y_ND) increased. MV led to a significant decline and accession of yield of regulated and non-regulated energy Y_NPQ and Y_NO, respectively. Membrane integrity and ATPase activity were reduced by HH stress, and DCMU and MV enhanced inhibitory actions.

The protective effects of CEF and LEF were mediated to a certain degree by meliorations in energy absorption and distribution as well as by maintenance of thylakoid membrane integrity and ATPase activity.