Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Therapy00:59

Gene Therapy

27.8K
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
27.8K
Recombinant DNA01:09

Recombinant DNA

103.9K
Overview
103.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same journal

Improving the precision of AAV lung gene therapy for SP-B deficiency using computationally derived lung-specific promoters.

Gene therapy·2026
Same journal

Recent advancements in improving cross-species applicability of bioengineered AAV capsids.

Gene therapy·2026
Same journal

Assessment of F/HN-pseudotyped lentiviral vector following intravenous delivery to mice.

Gene therapy·2026
Same journal

Applications of genome editing technologies in the treatment of human diseases.

Gene therapy·2026
Same journal

High resolution ES-DMA quantifies AAV capsid DNA content by electrical mobility to mass correlation.

Gene therapy·2026
Same journal

AAV8-mediated mouse/human PROC expression rescues thrombophilia in hereditary protein C-deficient mice.

Gene therapy·2026
See all related articles

Related Experiment Video

Updated: Feb 27, 2026

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles
08:35

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles

Published on: January 4, 2012

28.8K

Recombinant elastin-based nanoparticles for targeted gene therapy.

D A Monfort1, P Koria1

  • 1Department of Chemical and Biomedical Engineering, University of South Florida, Tampa, FL, USA.

Gene Therapy
|July 7, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces a novel nanoparticle system for targeted gene delivery using lentiviral vectors. The heterogeneous nanoparticles enhance gene delivery to specific cells overexpressing growth factor receptors.

More Related Videos

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
11:46

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Published on: May 19, 2018

13.2K
Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles
09:01

Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles

Published on: September 27, 2013

11.6K

Related Experiment Videos

Last Updated: Feb 27, 2026

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles
08:35

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles

Published on: January 4, 2012

28.8K
Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
11:46

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Published on: May 19, 2018

13.2K
Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles
09:01

Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles

Published on: September 27, 2013

11.6K

Area of Science:

  • Biotechnology
  • Gene Therapy
  • Nanomedicine

Background:

  • Lentiviral vectors are efficient for gene delivery but lack specificity.
  • Nonspecific gene delivery can lead to adverse effects.
  • Targeted delivery systems are needed to improve lentiviral vector efficacy and safety.

Purpose of the Study:

  • To develop a heterogeneous nanoparticle (NP) system for targeted lentiviral particle delivery.
  • To investigate the efficacy of NPs composed of low-density lipoprotein receptor repeat 3 (LDLR3) and keratinocyte growth factor (KGF) for gene delivery.

Main Methods:

  • Constructed heterogeneous NPs incorporating LDLR3-ELP and KGF-ELP.
  • Assessed viral transduction efficiency in cells with varying KGF receptor expression levels.
  • Compared the performance of homogeneous and heterogeneous NP systems.

Main Results:

  • Homogeneous NPs (LDLR3-ELP only) inhibited viral transduction.
  • Heterogeneous NPs (KGF-ELP and LDLR3-ELP) significantly enhanced viral transduction.
  • Enhanced transduction was observed in cells with high KGF receptor expression.

Conclusions:

  • Heterogeneous NPs offer a promising strategy for targeted lentiviral gene delivery.
  • This system can specifically target cells overexpressing growth factor receptors, such as KGF receptors.
  • The developed NP system holds potential for improving therapeutic gene delivery applications.