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Related Experiment Video

Updated: Feb 27, 2026

Intravital Imaging of the Mouse Popliteal Lymph Node
08:41

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Murine chronic lymph node window for longitudinal intravital lymph node imaging.

Eelco F J Meijer1, Han-Sin Jeong2, Ethel R Pereira1

  • 1Edwin L. Steele Laboratories, Department of Radiation Oncology, and Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.

Nature Protocols
|July 7, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a chronic lymph node window (CLNW) for long-term intravital microscopy in mice. This innovation enables detailed study of immune responses and cancer metastasis within lymph nodes over 14 days.

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Related Experiment Videos

Last Updated: Feb 27, 2026

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Area of Science:

  • Immunology
  • Oncology
  • Surgical Innovation

Background:

  • Chronic imaging windows are crucial for studying organ function and disease in mice.
  • Long-term intravital imaging of lymph nodes is needed to understand immune responses and cancer metastasis.

Purpose of the Study:

  • To describe a novel chronic lymph node window (CLNW) surgical preparation for intravital microscopy of the murine inguinal lymph node.
  • To enable stable, longitudinal imaging of lymph node dynamics without repeated surgeries.

Main Methods:

  • Custom-made titanium CLNW incorporating a coverslip.
  • Surgical procedure allowing imaging for up to 14 days.
  • Development of a specialized imaging stage to minimize movement artifacts.

Main Results:

  • The CLNW allows stable, longitudinal intravital imaging of lymph nodes for up to 14 days.
  • The preparation preserves essential lymph node blood and lymph flow.
  • The procedure is efficient, taking approximately 30 minutes per mouse.

Conclusions:

  • The CLNW is a valuable tool for studying cellular behavior in lymph nodes during immune responses and cancer progression.
  • This technique facilitates long-term intravital imaging, advancing research in immunology and oncology.
  • Requires small-animal surgery expertise for successful implementation.