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Entosis Is Induced by Glucose Starvation
Jens C Hamann1, Alexandra Surcel2, Ruoyao Chen3
1Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Louis V. Gerstner, Jr. Graduate School of Biomedical Sciences, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
Cell Reports
|July 7, 2017
Summary
Glucose withdrawal triggers entosis, a cell death process where cells engulf neighbors. This metabolic stress response, involving AMP-activated protein kinase (AMPK), aids survival by nutrient recovery.
Area of Science:
- Cell Biology
- Cancer Biology
- Metabolism
Background:
- Entosis is a cell death mechanism involving neighbor cell ingestion, observed in cancers and driven by mechanical differences.
- While matrix detachment can induce entosis, other stressors activating this process remain largely unknown.
Purpose of the Study:
- To investigate if metabolic stressors, specifically glucose withdrawal, can induce entosis in adherent cells.
- To elucidate the role of AMP-activated protein kinase (AMPK) in glucose withdrawal-induced entosis.
Main Methods:
- Induction of entosis in adherent cells via glucose withdrawal.
- Analysis of cell deformability and AMP-activated protein kinase (AMPK) activity.
- Investigating the mechanism of cell elimination and its impact on winner cell proliferation.
Main Results:
- Glucose withdrawal induces entosis in adherent cells.
- Cell stiffness, regulated by AMP-activated protein kinase (AMPK), determines which cells are eliminated.
- Elimination of 'loser' cells via entosis supports 'winner' cell proliferation during nutrient deprivation.
Conclusions:
- Entosis is activated by metabolic stress (glucose withdrawal) in adherent cells.
- AMPK-dependent mechanical changes mediate cell elimination during nutrient stress.
- Entosis facilitates nutrient recovery and survival under metabolic stress.
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