Related Experiment Video
Updated: Feb 27, 2026

The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport
Published on: March 6, 2015
Roe-derived phospholipid administration enhances lymphatic docosahexaenoic acid-containing phospholipid absorption in
Kaeko Murota1, Mika Takagi1, Yomi Watanabe2
1Department of Life Science, Faculty of Science and Engineering, Kindai University, Japan.
Abstract:
Plasma n-3 fatty acids are important as the supplying pool of n-3 fatty acids to various tissues including the brain, although the relationship between dietary n-3 fatty acids and their molecular species in the plasma are not fully clarified. We investigated the intestinal absorption of docosahexaenoic acid (DHA) derived from fish roe phospholipid (Roe-PL) and compared it with fish oil triacylglycerol and free DHA using unanesthetized lymph-cannulated rats. The DHA absorption from intraduodenally administered three samples were not significantly different, whereas Roe-PL administration resulted in a significantly higher level of DHA in the phospholipid fraction than the other two samples administrations. DHA in Roe-PL at the sn-2 position was less hydrolyzed by pancreatin than by purified phospholipase A2 in vitro and simultaneous administration of free DHA and lysophosphatidylcholine did not produce the same results as the Roe-PL administration. Our results indicate that dietary DHA-containing phospholipid is effective to increase the systemic DHA incorporated into phospholipids via intestinal absorption and biosynthesis.
More Related Videos
06:14The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion
Published on: November 30, 2022
06:25Long-Term Catheterization of the Intestinal Lymph Trunk and Collection of Lymph in Neonatal Pigs
Published on: March 5, 2016
Related Concept Videos
Lipid Absorption
These breakdown products bind with bile salts and lecithin to form micelles, which quickly pass between microvilli to come in close contact with the apical...
Bioavailability Enhancement: Drug Permeability Enhancement
Methods for Studying Drug Absorption: In situ
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
Lipid Digestion