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Bombyx mori protein BmREEPa and BmPtchd could form a complex with BmNPV envelope protein GP64

Xiao-Long Dong1, Yun-Fei Wu2, Tai-Hang Liu2

  • 1State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, PR China; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China.

Biochemical and Biophysical Research Communications
|July 8, 2017
PubMed

Insights

Bombyx mori receptor expression enhancing protein a (BmREEPa) interacts with Bombyx mori patched domain containing protein (BmPtchd) to form a complex, potentially explaining Bombyx mori Nucleopolyhedrovirus budded virus invasion mechanisms.

Area of Science:

  • Molecular Biology
  • Virology
  • Insect Pathology

Background:

  • Bombyx mori receptor expression enhancing protein a (BmREEPa) is implicated in Bombyx mori Nucleopolyhedrovirus (BmNPV) budded virus (BV) invasion.
  • The precise molecular mechanism of BmREEPa's role in BV invasion remains elusive.

Purpose of the Study:

  • To identify proteins interacting with BmREEPa.
  • To elucidate the mechanism of BmREEPa in BmNPV BV invasion.

Main Methods:

  • Co-immunoprecipitation was employed to screen for BmREEPa interacting proteins.
  • Protein complex formation and expression levels were analyzed.

Main Results:

  • A novel interacting protein, Bombyx mori patched domain containing protein (BmPtchd), was identified.
  • BmPtchd, BmREEPa, and Glycoprotein 64 were found to form a ternary protein complex.
  • Cellular cholesterol levels influence the expression of BmREEPa and BmPtchd.

Conclusions:

  • BmPtchd is a key interacting partner of BmREEPa in the context of BmNPV BV invasion.
  • The formation of a BmPtchd-BmREEPa-Glycoprotein 64 complex provides a mechanistic basis for BV entry.
  • Cholesterol homeostasis may play a regulatory role in BmNPV infection.
Keywords:
BmPtchdBmREEPaCholesterolGP64Interacting

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