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Functional characterization and subcellular localization of miiuy croaker cytosolic MITA involved in activation NF-κB

Junxia Cui1, Qiong Yang1, Weihua Song1

  • 1Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.

Fish & Shellfish Immunology
|July 8, 2017
PubMed

Insights

Stimulator of interferon genes (STING) protein, also known as MITA, plays a key role in innate immunity. This study characterized miiuy croaker MITA (mmiMITA), finding it crucial for immune responses in fish.

Area of Science:

  • Immunology
  • Molecular Biology
  • Fish Biology

Background:

  • Pattern recognition receptors are vital for host defense against pathogens.
  • Stimulator of interferon genes (STING) protein, also known as MITA, is a critical endoplasmic reticulum adaptor in innate immunity.

Purpose of the Study:

  • To characterize the bioinformatics and functions of MITA from miiuy croaker (mmiMITA).
  • To investigate the role of mmiMITA in innate immune responses in teleost fish.

Main Methods:

  • Bioinformatic analysis of mmiMITA.
  • Gene expression analysis in miiuy croaker tissues after poly(I:C) stimulation.
  • Immunofluorescence assays in Hela cells.
  • NF-κB reporter gene assays to assess mmiMITA activity.

Main Results:

  • MmiMITA is ubiquitously expressed in miiuy croaker tissues, with highest levels in the liver.
  • Poly(I:C) stimulation significantly upregulated mmiMITA expression in liver, spleen, and kidney.
  • Transcriptome analysis confirmed mmiMITA's high sensitivity to poly(I:C) in vivo.
  • MmiMITA localizes to the cytoplasm and activates the NF-κB pathway upon overexpression.

Conclusions:

  • MmiMITA plays a significant role in the innate immune response of miiuy croaker, likely by activating NF-κB.
  • This research expands the understanding of teleost fish MITA and contributes to fish immunology research.
Keywords:
ExpressionImmune responsesMITANF-κBSubcellular localization

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