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Shinya Sakuma1, Yusuke Kasai, Takeshi Hayakawa

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Summary

This study introduces a novel on-chip cell sorting method using local-flow control. It achieves high-throughput sorting of both large and small cells, overcoming limitations of existing technologies.

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Sorting

Background:

  • Conventional on-chip cell sorting struggles with high-throughput sorting of large cells.
  • Existing methods face challenges in controlling high-speed flow over large sorting areas.

Purpose of the Study:

  • To develop a high-throughput on-chip cell sorting technique for both large and small cells.
  • To overcome the throughput-size trade-off inherent in current cell sorters.

Main Methods:

  • Utilized high-speed local-flow control with dual membrane pumps and piezoelectric actuators on a microfluidic chip.
  • Evaluated flow profile controllability and cell sorting efficacy for different cell sizes.

Main Results:

  • Successfully sorted large cells (up to 150 μm) at an equivalent throughput of 31 kHz.
  • Achieved high throughput, purity, and viability for both Euglena gracilis (23 kHz, 92.8% success) and GCIY cancer cells (11 kHz, 97.8% success).

Conclusions:

  • The proposed local-flow control method effectively sorts cells of varying sizes at high throughput.
  • This technique overcomes the throughput-size limitations of conventional on-chip cell sorters.