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Related Experiment Videos

[Adhesion in Corynebacterium diphtheriae].

N N Kostiukova, N A Pereverzev

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |November 1, 1985
    PubMed
    Summary

    This study found that Corynebacterium diphtheriae strains can cause sheep red blood cells to clump. Toxigenic strains from patients showed the strongest clumping, indicating differences in bacterial adhesion properties.

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    Area of Science:

    • Microbiology
    • Bacterial Pathogenesis
    • Immunology

    Background:

    • Corynebacterium diphtheriae is the causative agent of diphtheria.
    • Bacterial adhesion plays a crucial role in colonization and pathogenesis.
    • Sheep red blood cells (SRBC) are commonly used in immunological assays.

    Purpose of the Study:

    • To investigate the ability of different Corynebacterium diphtheriae strains to induce agglutination of sheep red blood cells (SRBC).
    • To compare the adhesive properties of toxigenic and nontoxigenic C. diphtheriae strains.
    • To determine if fimbriae are involved in the observed adhesion.

    Main Methods:

    • Cultivation and characterization of 8 C. diphtheriae strains of varying origins and toxigenicity.
    • Assay for the induction of agglutination of trypsinized SRBC by bacterial strains.
    • Electron microscopy to examine bacterial cell surfaces for fimbriae.

    Main Results:

    • All 8 studied C. diphtheriae strains induced agglutination of SRBC.
    • Toxigenic C. diphtheriae gravis strains isolated from patients exhibited higher SRBC adhesion than those from carriers.
    • Nontoxigenic C. diphtheriae mitis strains showed the lowest SRBC adhesion.
    • Electron microscopy did not detect fimbriae on the bacterial cell surfaces.

    Conclusions:

    • C. diphtheriae strains possess the ability to agglutinate SRBC, suggesting a general adhesive property.
    • Adhesion to SRBC varies among C. diphtheriae strains, with clinical isolates showing greater activity.
    • The mechanism of SRBC agglutination by these strains does not appear to involve fimbriae.

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