Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

24.4K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.4K
MicroRNAs01:22

MicroRNAs

4.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.5K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.5K
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion01:27

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion

2.8K
The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are...
2.8K
Insulin Secretory Vesicles01:05

Insulin Secretory Vesicles

7.1K
Insulin secretory vesicles release insulin to stimulate blood glucose uptake and regulate carbohydrate metabolism. When the blood glucose levels increase, glucose enters the pancreatic β-islet cells through glucose transporters. Once inside, glucose is metabolized through glycolysis, the citric acid cycle, and the electron transport chain, producing ATP. This increase in ATP concentration closes ATP-sensitive potassium channels, leading to depolarization of the membrane and the opening of...
7.1K
Insulin: The Receptor and Signaling Pathways01:28

Insulin: The Receptor and Signaling Pathways

4.7K
Insulin action is mediated through a receptor tyrosine kinase, akin to the IGF-1 receptor. The number of receptors per cell varies significantly, from 40 on erythrocytes to 300,000 on adipocytes and hepatocytes. The insulin receptor consists of linked α/β subunit dimers, forming a heterotetramer glycoprotein with two extracellular α subunits and two β subunits spanning the membrane. The α subunits inhibit the inherent tyrosine kinase activity of the β subunits, but...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unveiling novel biomarkers for diabetes-related complications through large-scale proteomics analysis: A FIELD sub-study.

Journal of diabetes and its complications·2026
Same author

High-Content SRS Imaging Unveils Altered Cholesterol Metabolism in Ovarian Cancers Under CAR-T Treatment.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Nanozyme Aptasensor Array for Predictive Sensing of Virulent and Antibiotic-Resistant Staphylococcus Aureus strains.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

What do you most hope we will achieve with mammalian synthetic biology within the next decade?

Cell systems·2025
Same author

Plasma multi-omics and machine learning reveal predictive biomarkers for type 2 diabetes and retinopathy in Qatar biobank cohort.

Journal of translational medicine·2025
Same author

Cholesterol metabolism modulation facilitates CAR-T induced killing of ovarian cancer.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Feb 27, 2026

Visualization of Endogenous Mitophagy Complexes In Situ in Human Pancreatic Beta Cells Utilizing Proximity Ligation Assay
08:40

Visualization of Endogenous Mitophagy Complexes In Situ in Human Pancreatic Beta Cells Utilizing Proximity Ligation Assay

Published on: May 2, 2019

6.4K

Connexins and microRNAs: Interlinked players in regulating islet function?

Malati R Umrani1,2, Mugdha V Joglekar2, Ella Somerville Glover2

  • 1a National centre for cell science , Ganeshkhind, Pune University Campus , Pune , India.

Islets
|July 8, 2017
PubMed
Summary

This study explores the link between microRNAs (miRNAs) and connexin 36 (Cx36) in pancreatic beta-cells. We propose that miRNAs regulate Cx36, impacting coordinated insulin secretion.

Keywords:
connexinsinsulinintercellular communicationisletsmicroRNAs

More Related Videos

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol
08:04

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol

Published on: November 3, 2023

2.6K
MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
12:21

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

Published on: November 30, 2013

15.4K

Related Experiment Videos

Last Updated: Feb 27, 2026

Visualization of Endogenous Mitophagy Complexes In Situ in Human Pancreatic Beta Cells Utilizing Proximity Ligation Assay
08:40

Visualization of Endogenous Mitophagy Complexes In Situ in Human Pancreatic Beta Cells Utilizing Proximity Ligation Assay

Published on: May 2, 2019

6.4K
Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol
08:04

Author Spotlight: Investigating Islet Abnormalities and Function with a Pseudoislet Protocol

Published on: November 3, 2023

2.6K
MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
12:21

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

Published on: November 30, 2013

15.4K

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Cell Biology

Background:

  • Pancreatic beta-cells communicate via gap junctions, with Connexin 36 (Cx36) being crucial for coordinated insulin secretion.
  • MicroRNAs (miRNAs) are key regulators of cellular processes, but their role in connexin regulation is understudied.
  • The interplay between miRNAs and Cx36 in islet function remains largely unexplored.

Purpose of the Study:

  • To review and discuss the potential role of miRNAs in regulating Cx36 expression.
  • To highlight the significance of this regulation for pancreatic islet function and insulin secretion.

Main Methods:

  • Literature review and synthesis of existing research on connexins, miRNAs, and islet function.

Main Results:

  • While direct evidence is lacking, the known functions of miRNAs and connexins suggest a regulatory relationship.
  • Cx36 expression is likely influenced by specific miRNAs, impacting beta-cell communication.

Conclusions:

  • There is a plausible mechanistic link between miRNAs and Cx36 in pancreatic islets.
  • Further research is warranted to elucidate these pathways and their implications for metabolic diseases.