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Micro-CT and FE-SEM enamel analyses of calcium-based agent application after bleaching
Mauricio Neves Gomes1, Flávia Pires Rodrigues2,3, Nick Silikas4
1Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, Av. Prof. Lineu Prestes, 2227, Cidade Universitária, São Paulo, SP, 05508-900, Brazil. maungomes@gmail.com.
Clinical Oral Investigations
|July 10, 2017
Summary
Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) paste applied for 7 days significantly increased enamel thickness and calcium content after bleaching. This treatment neutralizes adverse effects and minimizes side effects of dental bleaching.
Area of Science:
- Biomaterials Science
- Dental Materials
- Cariology
Background:
- Dental bleaching with hydrogen peroxide can cause enamel demineralization.
- Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) is known for its remineralizing properties.
Purpose of the Study:
- To evaluate the effects of CPP-ACP on bleached enamel.
- To assess the structural and mineral content changes in enamel after bleaching and CPP-ACP application.
Main Methods:
- Premolar teeth were bleached with 35% hydrogen peroxide.
- A CPP-ACP paste was applied for 7 days.
- Microtomography and SEM EDX were used to analyze enamel structure and mineral content.
Main Results:
- Significant increase in enamel structure thickness and calcium content.
- Decreased separation and fragmentation index in the outer enamel (56.2 μm depth).
- Formation of a covering layer and reduced inter-crystal spaces observed.
Conclusions:
- CPP-ACP application creates a compact enamel structure due to calcium deposition.
- CPP-ACP neutralizes adverse effects and minimizes side effects of dental bleaching.
- CT parameters are valuable for assessing enamel mineralization and remineralization.
