Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Structure01:23

RNA Structure

79.5K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
79.5K
RNA Structure01:19

RNA Structure

7.9K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
7.9K
Protein Folding01:25

Protein Folding

11.9K
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
11.9K
Protein Folding01:22

Protein Folding

128.9K
Overview
128.9K
RNA Stability01:53

RNA Stability

35.9K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.9K
Nucleic Acid Structure01:25

Nucleic Acid Structure

9.7K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
9.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

LiraSearch-ultrafast ligand shape and electrostatic matching server.

Bioinformatics advances·2026
Same author

Ten common misconceptions about Galaxy (and why they are wrong!).

PLoS computational biology·2026
Same author

Standardizing RNA-seq Analysis of Fungal Pathogens Using BRC-Analytics and Agentic AI: A <i>Candidozyma auris</i> Case Study.

bioRxiv : the preprint server for biology·2026
Same author

From data to publication in a browser with BRC-Analytics: Evolutionary dynamics of coding overlaps in measles virus.

bioRxiv : the preprint server for biology·2025
Same author

Fibroblast growth factor signaling induces a chondrocyte-like state of peripheral nerve fibroblast during aging.

Nature communications·2025
Same author

PathoGFAIR: a collection of FAIR and adaptable (meta)genomics workflows for (foodborne) pathogens detection and tracking.

GigaScience·2025
Same journal

The Effect of Reduced Graphene Oxide in Chitosan-Based Nanoparticles on the Enzymatic Properties of the Immobilized Enzyme.

Journal of biotechnology·2026
Same journal

High-level biosynthesis of gastrodin in engineered Escherichia coli.

Journal of biotechnology·2026
Same journal

From plasmid sequence to process design: A computational analysis of metabolism in the context of plasmid DNA manufacturing.

Journal of biotechnology·2026
Same journal

Development of an inducible cellobiohydrolase promoter and its application for enhancing the production of ganoderic acids in Ganoderma lingzhi.

Journal of biotechnology·2026
Same journal

Malic acid and allied exogenous chemicals induces desaturase gene expression and elevates PUFA production in marine microalgae Isochrysis sp.

Journal of biotechnology·2026
Same journal

Recombinant production of human papillomavirus type 16 E6 and E7 vaccine antigens in Chlamydomonas reinhardtii.

Journal of biotechnology·2026
See all related articles

Related Experiment Video

Updated: Feb 27, 2026

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

15.5K

Recent advances in RNA folding.

Jörg Fallmann1, Sebastian Will2, Jan Engelhardt1

  • 1Bioinformatics Group, Department of Computer Science; and Interdisciplinary Center for Bioinformatics, University of Leipzig, Härtelstraße 16-18, D-04107 Leipzig, Germany.

Journal of Biotechnology
|July 11, 2017
PubMed
Summary
This summary is machine-generated.

RNA secondary structures are crucial for function and evolution, offering insights into biological processes. This study reviews computational methods for analyzing RNA folding, alignment, and comparison, highlighting key software tools.

Keywords:
RNA analysisRNA constraint foldingRNA interactionsRNA secondary structureRNA secondary structure comparison

More Related Videos

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

5.9K
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

5.3K

Related Experiment Videos

Last Updated: Feb 27, 2026

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

15.5K
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

5.9K
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

5.3K

Area of Science:

  • Computational Biology
  • Molecular Biology
  • Bioinformatics

Background:

  • RNA secondary structure is a key intermediate in nucleic acid structure, significantly contributing to free energy.
  • Secondary structures exhibit strong evolutionary conservation, often evolving slower than primary sequences.
  • The link between RNA structure and function makes secondary structure analysis essential for interpreting experimental data.

Purpose of the Study:

  • To provide an overview of RNA folding algorithms and their recent advancements.
  • To discuss methods for aligning, comparing, and clustering RNA secondary structures.
  • To present a summary of prominent software suites for RNA structure analysis.

Main Methods:

  • Review of existing RNA folding algorithms, including dynamic programming approaches.
  • Exploration of computational techniques for RNA structure comparison and classification.
  • Compilation and tabular summary of relevant bioinformatics software.

Main Results:

  • RNA secondary structures are computationally tractable and evolutionarily conserved.
  • High-throughput methods now enable direct experimental assay of secondary structures.
  • A comprehensive overview of computational tools for RNA structure analysis is presented.

Conclusions:

  • Computational analysis of RNA secondary structures is vital for understanding their function and evolution.
  • The reviewed algorithms and software facilitate efficient analysis of large-scale RNA structure data.
  • This work serves as a guide to the current landscape of RNA folding and structure analysis tools.