Related Experiment Video
Updated: Feb 27, 2026
Identification of Mycobacterium Species by DNA Microarray Chip Method
Published on: June 24, 2025
[Diagnostic value of different detection methods for Mycobacterium tuberculosis in bronchoalveolar lavage fluid from
Shali Jiang1, Weijun Liang2, Demao Zhu3
1Department of Pathology, Changsha Central Hospital, Changsha 410004, China 943202965@qq.com.
Objective:
To investigate the diagnostic value of different detection methods for Mycobacterium tuberculosis in bronchoalveolar lavage fluid (BALF) from patients with pulmonary tuberculosis. Methods: BALF from100 patients in Changsha Central Hospital from January 2013 to December 2015 was collected. Among 100 patients, 65 cases were clinically diagnosed as tuberculosis, and 35 cases served as control. BALF smear method, polymerase chain reaction (PCR) and membrane reverse dot blot (RDB) were used for synchronous detection of Mycobacterium tuberculosis. Results: The positive rates by BALF smear method, PCR and RDB were 43.08%, 73.84% and 92.31%, respectively (P<0.05). Sensitivity, specificity, accuracy, and negative predictive value for BALF smear were 43.08%, 88.57%, 59.00%, and 45.59%, respectively; for PCR were 73.85%, 100%, 83.00%, and 67.31%, respectively; for RDB were 92.31%, 100.00%, 95.00%, and 87.50%, respectively. Conclusion: The technique of membrane RDB can not only accurately diagnose Mycobacterium tuberculosis, but also can rapidly and easily identify the resistance of Mycobacterium tuberculosis to streptomycin (SM), rifampicin (RFP) and isoniazid (INH) genotypes. It possesses high clinical value.
