Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characterization of opioid receptor subtypes in solution.

C D Demoliou-Mason, E A Barnard

    Journal of Neurochemistry
    |April 1, 1986
    PubMed
    Summary

    Stable opioid receptor binding activity was successfully extracted and characterized. Magnesium ions are crucial for high-affinity binding of opioid peptides to soluble mu, delta, and kappa opioid receptors.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The P2Y nucleotide receptors in the human genome.

    Acta biologica Hungarica·2003
    Same author

    Expression of the P2Y1 nucleotide receptor in chick muscle: its functional role in the regulation of acetylcholinesterase and acetylcholine receptor.

    The Journal of neuroscience : the official journal of the Society for Neuroscience·2001
    Same author

    An elusive receptor is finally caught: P2Y(12'), an important drug target in platelets.

    Trends in pharmacological sciences·2001
    Same author

    Adenosine nucleotides acting at the human P2Y1 receptor stimulate mitogen-activated protein kinases and induce apoptosis.

    The Journal of biological chemistry·2001
    Same author

    Structural diversity despite strong evolutionary conservation in the 5'-untranslated region of the P-type dystrophin transcript.

    Molecular and cellular neurosciences·2001
    Same author

    Dual effect of nucleotides on P2Y receptors.

    IUBMB life·2001

    Area of Science:

    • Neuroscience
    • Pharmacology
    • Biochemistry

    Background:

    • Opioid receptors are critical targets for pain management and addiction therapies.
    • Understanding the properties of soluble opioid receptors is essential for drug development.

    Purpose of the Study:

    • To obtain stable, soluble opioid receptor preparations with preserved subtype specificities.
    • To investigate the influence of divalent cations, particularly Mg2+, on opioid receptor binding characteristics.

    Main Methods:

    • Rat brain membranes were preincubated with Mg2+ and then solubilized using digitonin.
    • Opioid receptor binding assays were performed using radioligands for opioid peptides and alkaloids.
    • Size exclusion chromatography was used to determine the molecular size of soluble receptor complexes.

    Main Results:

    • High yields of stable, digitonin-solubilized opioid receptors retaining mu, delta, and kappa subtype specificities were achieved.
    • Magnesium ions significantly enhanced high-affinity and high-capacity binding of opioid peptides to soluble receptors.
    • Soluble opioid receptor binding exhibited similar sensitivities to Na+ and guanine nucleotides as membrane-bound receptors.
    • Ligand-receptor interactions suggested positive cooperativity, possibly due to receptor subunit association/dissociation.

    Conclusions:

    • Magnesium ions are vital for maintaining the functional integrity and high-affinity binding of soluble opioid receptors, especially for opioid peptides.
    • The study provides a valuable tool for further investigation into opioid receptor pharmacology and the development of novel therapeutics.

    Related Experiment Videos