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Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
Published on: October 9, 2020
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Transcriptomic profile analysis of mouse neural tube development by RNA-Seq
Juan Yu1, Jianbing Mu2, Qian Guo1
1Department of Biochemistry and Molecular Biology, Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Shanxi Medical University, Taiyuan, China.
IUBMB Life
|July 11, 2017
Summary
This study reveals key genes and alternative splicing events in mouse neural tube development. Findings offer insights into neural tube defects (NTDs) and potential therapeutic targets.
Area of Science:
- Developmental Biology
- Neuroscience
- Genomics
Background:
- Neural tube development is crucial for central nervous system (CNS) formation but remains incompletely understood.
- Understanding neural tube development mechanisms is vital for elucidating neural tube defects (NTDs).
Purpose of the Study:
- To investigate gene expression profiles during mouse neural tube development.
- To identify differentially expressed genes (DEGs) and alternative splicing events.
- To explore the role of these genes in neural tube defects (NTDs).
Main Methods:
- RNA sequencing (RNA-seq) was employed to analyze gene expression at embryonic days (E) 8.5, 9.5, and 10.5.
- Bioinformatic analysis was used to screen for differentially expressed genes and alternative splicing events.
- Comparison with a retinoic acid-induced NTDs model was performed.
Main Results:
- 391 differentially expressed genes (DEGs) were identified during mouse neural tube development.
- 45 DEGs were associated with CNS development, with Bmp2, Ascl1, Olig2, Lhx1, Wnt7b, and Eomes highlighted.
- Alternative splicing was observed in 10 neural-related genes, and seven neural splicing factors were differentially expressed. Nine DEGs were dysregulated in an NTDs model.
Conclusions:
- This study provides comprehensive gene expression and alternative splicing data for mouse neural tube development.
- Identified genes and splicing events offer potential insights into the molecular basis of NTDs.
- The findings may contribute to understanding NTDs pathogenesis and developing diagnostic or therapeutic strategies.
