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Single Cell Level Quantification of Nanoparticle-Cell Interactions Using Mass Cytometry.

Angela Ivask^1,2, Andrew J Mitchell³, Christopher M Hope⁴

¹Future Industries Institute, University of South Australia , Mawson Lakes, Australia.

Analytical Chemistry

|July 11, 2017

Summary

This summary is machine-generated.

Mass cytometry enables quantitative, single-cell analysis of nanoparticle (NP) interactions within human T-lymphocytes. This approach reveals significant heterogeneity in silver nanoparticle (Ag NP) uptake and extracellular binding, crucial for nanomedicine and nanotoxicology.

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Area of Science:

Nanotoxicology
Nanomedicine
Biotechnology

Background:

Quantifying cell-associated nanoparticles (NPs) is critical for nanomedicine and nanotoxicology.
Traditional bulk analysis methods like ICP-MS struggle with single-cell resolution.
Understanding NP-cell interactions at the individual cell level is essential.

Purpose of the Study:

To quantitatively assess extra- and intracellular silver (Ag) in individual human T-lymphocytes exposed to Ag NPs using mass cytometry.
To investigate the heterogeneity of Ag NP interactions at the single-cell level.
To evaluate mass cytometry as a tool for single-cell NP quantification.

Main Methods:

Utilized mass cytometry, combining single-cell analysis with time-of-flight mass spectrometry.
Exposed human T-lymphocytes to various doses, sizes, and surface coatings of silver (Ag) nanoparticles.
Quantified extracellularly bound Ag and intracellularly uptaken Ag at the single-cell level.

Main Results:

Demonstrated significant population heterogeneity in both extracellular Ag binding and cellular uptake of Ag NPs.
Observed wide variations in Ag quantification (e.g., extracellularly bound Ag from 79 to 560 fg; cellular uptake from 17 to 121 fg) in cells exposed to 3 μg/mL of 30 nm branched polyethylene imine coated Ag NPs.
Similar heterogeneity was noted across different Ag NP doses, sizes, and surface coatings.

Conclusions:

Mass cytometry provides a powerful method for quantitative, single-cell analysis of nanoparticle-cell interactions.
Significant heterogeneity exists in NP association and uptake by individual cells, highlighting the limitations of bulk analysis.
Despite limitations (e.g., inability to assess NP transformation), mass cytometry is a promising technique for advancing the field of NP-cell interaction studies.