Related Experiment Video

Updated: Feb 27, 2026

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
12:18

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

Published on: February 1, 2020

6.2K

Correlation study of DNA methylation of WNT6 gene with osteosarcoma in children

Li Li1, Chi Xu2, Pingtao Liu1

  • 1Department I of Orthopedics, Jingmen No. 2 People's Hospital, Jingmen, Hubei 448000, P.R. China.

Oncology Letters
|July 12, 2017
PubMed

Insights

DNA methylation of the WNT6 gene is linked to childhood osteosarcoma. Lower WNT6 gene methylation correlates with poorer prognosis in pediatric osteosarcoma patients, indicating a potential biomarker.

Area of Science:

  • Oncology
  • Epigenetics
  • Pediatric Cancer Research

Background:

  • Osteosarcoma is a primary bone cancer affecting children and adolescents.
  • DNA methylation plays a crucial role in gene regulation and cancer development.
  • The WNT6 gene's role in osteosarcoma, particularly concerning its epigenetic regulation, requires further investigation.

Purpose of the Study:

  • To assess the DNA methylation status of the WNT6 gene in pediatric osteosarcoma.
  • To explore the relationship between WNT6 gene methylation and osteosarcoma in children.
  • To determine the impact of WNT6 gene methylation on patient prognosis.

Main Methods:

  • Immunohistochemical staining for WNT6 protein expression in 50 pediatric osteosarcoma samples.
  • Methylation-specific PCR (MSP) to evaluate WNT6 gene DNA methylation levels.
  • Western blot analysis in osteosarcoma (MG63) and normal osteoblast (Hfob1.19) cell lines.
  • Statistical analysis of clinical and prognostic data to correlate WNT6 methylation with outcomes.

Main Results:

  • WNT6 protein was expressed in 84% of primary osteosarcoma cases.
  • DNA methylation of the WNT6 gene was detected in 18% of primary osteosarcoma samples.
  • Osteosarcoma cells exhibited higher WNT6 protein expression and lower WNT6 gene methylation compared to normal osteoblasts.
  • A negative correlation was found between WNT6 gene DNA methylation levels and the prognosis of children with osteosarcoma.

Conclusions:

  • High WNT6 gene expression in pediatric osteosarcoma is associated with low DNA methylation levels.
  • WNT6 gene DNA methylation is negatively correlated with the prognosis of children diagnosed with osteosarcoma.
  • WNT6 gene methylation status may serve as a prognostic biomarker for pediatric osteosarcoma.

Keywords:
MG63 cell lineWNT6gene alterationmethylationosteosarcoma

More Related Videos

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells05:27

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells

Published on: June 16, 2023

2.4K
Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies14:56

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Published on: May 6, 2022

5.3K

Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
4.0K
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
34.0K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
10.8K