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Updated: Feb 26, 2026

Encapsulation and Permeability Characteristics of Plasma Polymerized Hollow Particles
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What´s floating on my plasma?

Janne Cadamuro1, Helmut Wiedemann1, Thomas K Felder1

  • 1Department of Laboratory Medicine, Paracelsus Medical University, Salzburg, Austria.

Biochemia Medica
|July 12, 2017
PubMed
Summary

A preanalytical issue involving heparin-gel tubes caused implausible glucose results due to white particulate matter and gel droplets. These contaminants temporarily clogged pipetting needles, affecting sample volume and analysis outcomes.

Keywords:
case reportlithium-heparin tubesseparator gelwhite particulate matter

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Area of Science:

  • Clinical Chemistry
  • Laboratory Medicine
  • Preanalytical Variables

Background:

  • Routine clinical chemistry analyses are susceptible to preanalytical issues.
  • Heparin-gel tubes are commonly used for blood sample collection and analysis.

Observation:

  • An implausible low glucose value was initially measured.
  • Re-analysis yielded a normal glucose value.
  • Microscopic examination revealed white particulate matter (WPM) and fatty droplets in the plasma.

Findings:

  • The WPM comprised fibrinogen, platelets, and leukocytes.
  • Fatty droplets likely originated from the separator gel.
  • These contaminants formed a temporary clot in the instrument's pipetting needle, altering sample volume and glucose readings.

Implications:

  • Identified potential causes include high-speed centrifugation, high cholesterol, gel formulation, and improper tube storage.
  • Implementing instrument cleansing and changing tube batches resolved the issue.
  • This highlights the importance of addressing preanalytical variables for accurate clinical chemistry results.