Antimicrobial Resistant Pattern and Capsular Typing of Streptococcus Pneumoniae Isolated from Children in Sistan...

Ankyloglossia is an anatomic developmental anomaly determining by thick and short, fi brotic ferenum. Tongue changes have severe eff ects on occlusion and oropharyngeal structures. The aim of this study was to evaluate the position of hyoid in children 7-11 years` old with ankyloglossia in lateral cephalometric radiographs.

In this study 260 nasopharyngeal swabs were taken from non-vaccinated healthy children between 6 months to 6 years old at medical centers in Sistan-Baluchestan during August 2013 to January 2014. These samples were cultured on blood agar. Primary identifi cation of bacterial isolated was determined by biochemical analysis and molecular tests. Capsular typing was performed by Multiplex PCR using primers targeting cps locus that is highly conserved among diff erent capsular types. The master mixes for PCR were grouped them into six multiplex reactions.

Out of 260 nasopharyngeal swabs, 42 isolates of Streptococcus pneumoniae were detected and identifi ed. The overall pneumococcal carriage rate was 16.1%. The most frequently isolated capsular types were: 6A/B, 19A, 19F and 23F. These capsular types accounted for 49.9% of all strains detected.

We found that the prevalence of pneumococcal carriage among non-vaccinated children under six years old is about 16%. Our study provides much data about carriage rate and pneumococcal capsular types in preschool children, which is necessary for predicting the diff erent valent pneumococcal conjugated vaccines in Iran.