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Nucleotide sequence of the human N-myc gene
Summary
The N-myc gene, similar to the protooncogene c-myc, is amplified in neuroblastomas. Determining the N-myc sequence reveals striking structural and functional similarities to c-myc, supporting its role as a protooncogene.
Area of Science:
- Molecular biology
- Oncology
- Genetics
Background:
- Neuroblastomas often show amplified N-myc expression, suggesting its oncogenic potential.
- N-myc shares functional similarities with the protooncogene c-myc in cell culture.
Purpose of the Study:
- To elucidate the relationship between N-myc and c-myc.
- To determine the nucleotide and amino acid sequences of human N-myc.
Main Methods:
- Nucleotide sequencing of the human N-myc gene.
- Deduction of the encoded amino acid sequence.
Main Results:
- N-myc exhibits significant topographical similarity to c-myc.
- Both genes possess three similarly sized exons, with coding regions in exons two and three.
- Both mRNAs have long 5' untranslated regions, suggesting shared translational control mechanisms.
Conclusions:
- The structural resemblance between N-myc and c-myc proteins supports their related functions.
- N-myc is strongly implicated as a protooncogene involved in neuroblastoma development.