First study on iron complexes in blood and organ samples from thalassaemic and normal laboratory mice using Mössbauer...

Measurements of iron complexes and iron stores in the body are crucial for evaluation and management of chelation therapy targeted against iron accumulation or overload in blood and organs. In this work, blood and tissue samples from one normal and one thalassaemic laboratory mouse were studied using ⁵⁷Fe Mössbauer spectroscopy at 78 K for the first time. In contrast to human patients, these laboratory mice did not receive any medical treatment, thus the iron components present in the samples are not altered from their natural state. The Mössbauer spectra of blood, liver and spleen samples of the thalassaemic mouse were found to differ in shape and iron content compared with corresponding spectra of the normal mouse. These results demonstrate a basis for further exploitation of the thalassaemic mouse model to study thalassaemia and its treatment in more detail using Mössbauer spectroscopy.