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Methods to Assess the Role of Poly(ADP-Ribose) Polymerases in Regulating Mitochondrial Oxidation
Edit Mikó1,2, Tünde Kovács1, Tamás Fodor1
1Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, 4032 Egyetem tér 1, Debrecen, H-4032, Hungary.
Abstract:
The impact of poly(ADP-ribose) polymerase (PARP) enzymes on cellular NAD+ has been established for almost 30 years now and its sequel, the metabolic collapse of cells upon PARP overactivation is a nearly 20-year-old observation. However, in the last decade there was an enormous blooming in the understanding of the interplay between PARPs and mitochondria. Mitochondrial activity can be assessed by a comprehensive set of methods that we aim to introduce here.
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