Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

929
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
929

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chemiluminescence microscopy single cell imaging using esterase triggered 1,2-dioxetanes.

The Analyst·2026
Same author

Viscosity effects on the chemiluminescence emission of 1,2-dioxetanes in water.

Organic & biomolecular chemistry·2025
Same author

An economical fluorescent method for microplastic detection in soil samples.

Analytical methods : advancing methods and applications·2025
Same author

A silicon rhodamine 1,2-dioxetane chemiluminophore for <i>in vivo</i> near-infrared imaging.

Organic & biomolecular chemistry·2025
Same author

Multi-stage and multi-colour liquid crystal reflections using a chiral triptycene photoswitchable dopant.

Nature chemistry·2024
Same author

Ex Tenebris Lux: Illuminating Reactive Oxygen and Nitrogen Species with Small Molecule Probes.

Chemical reviews·2024

Related Experiment Video

Updated: Feb 26, 2026

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy
10:05

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy

Published on: May 22, 2020

11.2K

A volumetric three-dimensional digital light photoactivatable dye display.

Shreya K Patel1, Jian Cao1,2, Alexander R Lippert1,2,3

  • 1Department of Chemistry, Southern Methodist University, 3215 Daniel Avenue, Dallas, Texas 75275-0314, USA.

Nature Communications
|July 12, 2017
PubMed
Summary

Researchers developed a novel volumetric 3D display using photoactivatable dyes. This 3D Light PAD system creates detailed 3D images and animations, paving the way for accessible volumetric displays.

More Related Videos

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
11:34

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

16.1K
Pulsed Laser Diode-Based Desktop Photoacoustic Tomography for Monitoring Wash-In and Wash-Out of Dye in Rat Cortical Vasculature
06:46

Pulsed Laser Diode-Based Desktop Photoacoustic Tomography for Monitoring Wash-In and Wash-Out of Dye in Rat Cortical Vasculature

Published on: May 30, 2019

8.9K

Related Experiment Videos

Last Updated: Feb 26, 2026

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy
10:05

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy

Published on: May 22, 2020

11.2K
High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
11:34

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

16.1K
Pulsed Laser Diode-Based Desktop Photoacoustic Tomography for Monitoring Wash-In and Wash-Out of Dye in Rat Cortical Vasculature
06:46

Pulsed Laser Diode-Based Desktop Photoacoustic Tomography for Monitoring Wash-In and Wash-Out of Dye in Rat Cortical Vasculature

Published on: May 30, 2019

8.9K

Area of Science:

  • Optics and Photonics
  • Materials Science
  • Display Technology

Background:

  • Volumetric three-dimensional (3D) displays offer true 3D visualization with a 360° view.
  • Challenges in fabrication complexity have hindered widespread implementation of volumetric 3D displays.

Purpose of the Study:

  • To report a chemically enabled volumetric 3D digital light photoactivatable dye display (3D Light PAD).
  • To establish a foundation for widely accessible volumetric 3D display technology.

Main Methods:

  • Utilized photoactivatable dyes that exhibit reversible fluorescence upon ultraviolet light exposure.
  • Employed structured light beams and tuned dye kinetics and emission wavelengths to generate spatial fluorescence patterns.
  • Fabricated a first-generation 3D Light PAD using N-phenyl spirolactam rhodamine B, a picoprojector, an ultraviolet projector, and a quartz imaging chamber.

Main Results:

  • Achieved a minimum voxel size of 0.68 mm³ and a resolution of 200 μm.
  • Demonstrated good stability over repeated on-off cycles.
  • Successfully projected a range of high-resolution 3D images and animations.

Conclusions:

  • The 3D Light PAD system offers a viable approach to volumetric 3D display technology.
  • This chemically enabled method addresses fabrication complexities, enabling more accessible volumetric displays.