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Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

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Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
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Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Rapid Analysis and Exploration of Fluorescence Microscopy Images
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Rapid Analysis and Exploration of Fluorescence Microscopy Images

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Microscopy and Image Analysis.

George McNamara1, Michael Difilippantonio2, Thomas Ried3

  • 1Biomedical Consultant, Baltimore, Maryland.

Current Protocols in Human Genetics
|July 12, 2017
PubMed
Summary
This summary is machine-generated.

This review covers light microscopy techniques, including fluorescence microscopy and fluorescence in situ hybridization (FISH). It highlights emerging optical clearing and expansion microscopy methods for biomedical research.

Keywords:
digital imagingfluorescence in situ hybridizationfunctional genomicslight microscopy

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Area of Science:

  • Biomedical Imaging
  • Cell Biology
  • Microscopy Techniques

Background:

  • Light microscopy is fundamental in biomedical research.
  • Fluorescence microscopy and FISH are key techniques for cellular and genetic analysis.
  • Advancements in microscopy offer new possibilities for biological sample investigation.

Purpose of the Study:

  • To provide a comprehensive overview of light microscopy principles and applications.
  • To detail components such as objectives, light sources, filters, and photography.
  • To introduce emerging techniques like optical clearing and expansion microscopy.

Main Methods:

  • Review of established light microscopy components and techniques.
  • Discussion of color photography for fluorescence microscopy and FISH.
  • Brief introduction to specimen optical clearing and expansion microscopy.

Main Results:

  • Detailed explanation of light microscopy elements.
  • Guidance on photographic methods for fluorescence and FISH.
  • Identification of new opportunities in advanced microscopy.

Conclusions:

  • Light microscopy remains a vital tool in biomedical science.
  • Emerging techniques like optical clearing and expansion microscopy present significant potential.
  • Cytogeneticists and pathologists can benefit from adopting these advanced methods.