Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Culture01:21

Cell Culture

23.2K
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
23.2K
Cell Lines01:16

Cell Lines

10.6K
A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
10.6K
Plant Tissue Culture02:57

Plant Tissue Culture

40.9K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
40.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Restricted and Repetitive Behaviors in Intellectual Disabilities: A Scoping Review with a Systematic Search of the Literature.

Reviews on recent clinical trials·2026
Same author

Remote Language Assessment in School-Age Children With Phelan-McDermid Syndrome and Genotype-Phenotype Correlation.

American journal of medical genetics. Part A·2026
Same author

Behavioral Features in Phelan-McDermid Syndrome: Characteristics and Genetic and Metabolic Contributions in a Cohort of 56 Individuals.

Genes·2026
Same author

Craniofacial Dysmorphology Associated With Phelan-McDermid Syndrome Using Three-Dimensional Morphometrics.

Clinical genetics·2026
Same author

Metabolic Dysfunction-Associated Steatotic Liver Disease in a Patient with Phelan-McDermid Syndrome.

Life (Basel, Switzerland)·2025
Same author

Genetics of kidney disorders in Phelan-McDermid syndrome: evidence from 357 registry participants.

Pediatric nephrology (Berlin, Germany)·2023

Related Experiment Video

Updated: Feb 26, 2026

Trypsinizing and Subculturing Mammalian Cells
05:59

Trypsinizing and Subculturing Mammalian Cells

Published on: June 12, 2008

22.3K

Mammalian Cell Tissue Culture.

Katy Phelan1, Kristin M May2

  • 1Cytogenetics Laboratory, Florida Cancer Specialists & Research Institute, Fort Myers, Florida.

Current Protocols in Human Genetics
|July 12, 2017
PubMed
Summary

This study outlines essential techniques for preserving mammalian cell cultures, crucial for human genetics research. Mastering aseptic techniques, media preparation, and cell cryopreservation ensures reliable experimental outcomes.

Keywords:
aseptic techniquefreezing cellsmammalian cellsmediumpassaging cellstissue culture

More Related Videos

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
08:39

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

Published on: December 5, 2011

38.5K
A Three-dimensional Tissue Culture Model to Study Primary Human Bone Marrow and its Malignancies
08:51

A Three-dimensional Tissue Culture Model to Study Primary Human Bone Marrow and its Malignancies

Published on: March 8, 2014

17.2K

Related Experiment Videos

Last Updated: Feb 26, 2026

Trypsinizing and Subculturing Mammalian Cells
05:59

Trypsinizing and Subculturing Mammalian Cells

Published on: June 12, 2008

22.3K
Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
08:39

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

Published on: December 5, 2011

38.5K
A Three-dimensional Tissue Culture Model to Study Primary Human Bone Marrow and its Malignancies
08:51

A Three-dimensional Tissue Culture Model to Study Primary Human Bone Marrow and its Malignancies

Published on: March 8, 2014

17.2K

Area of Science:

  • Cell Biology
  • Human Genetics
  • Molecular Biology

Background:

  • Mammalian cell cultures are fundamental tools in human genetics research.
  • Maintaining cellular integrity, function, and viability in vitro is critical for accurate genetic studies.
  • Specialized skills are necessary for successful cell culture preservation.

Purpose of the Study:

  • To describe the fundamental skills required for maintaining and preserving mammalian cell cultures.
  • To provide a guide for researchers on essential cell culture techniques.
  • To ensure the reliability and reproducibility of experiments using cultured cells.

Main Methods:

  • Aseptic technique maintenance for contamination prevention.
  • Preparation of specialized cell culture media.
  • Cell passaging for subculturing and expansion.
  • Cryopreservation (freezing) and long-term storage protocols.
  • Recovery of frozen cell stocks.
  • Viable cell counting methodologies.

Main Results:

  • Detailed protocols for essential cell culture maintenance are presented.
  • The importance of aseptic technique is emphasized for preventing contamination.
  • Effective methods for cell freezing, storage, and recovery are described.
  • Techniques for ensuring cell viability and accurate counting are outlined.

Conclusions:

  • Proficiency in basic cell culture skills is vital for human genetics research.
  • Proper maintenance and preservation techniques ensure the integrity of cultured cells.
  • This unit serves as a foundational guide for researchers working with mammalian cell cultures.