Related Experiment Video

Updated: Feb 26, 2026

An Orthotopic Murine Model of Human Prostate Cancer Metastasis
06:48

An Orthotopic Murine Model of Human Prostate Cancer Metastasis

Published on: September 18, 2013

35.9K

The TGF-β signalling negative regulator PICK1 represses prostate cancer metastasis to bone

Yuhu Dai1,2, Dong Ren1,2, Qing Yang1,2

  • 1Department of Orthopaedic Surgery, The First Affiliated Hospital of Sun Yat-sen University, 58# Zhongshan 2rd Road, Guangzhou 510080, China.

British Journal of Cancer
|July 12, 2017
PubMed

Insights

Decreased PICK1 expression in prostate cancer (PCa) correlates with bone metastasis. Upregulating PICK1 inhibits PCa bone metastasis, revealing a new epigenetic mechanism involving miR-210-3p.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Metastasis

Background:

  • Constitutive activation of TGF-β signaling drives prostate cancer (PCa) bone metastasis.
  • Protein Interacting with PRKCA 1 (PICK1) acts as a negative regulator of the TGF-β pathway.
  • The role of PICK1 in PCa bone metastasis is not well understood.

Purpose of the Study:

  • To investigate the clinical significance of PICK1 in PCa bone metastasis.
  • To elucidate the biological role of PICK1 in PCa progression.
  • To identify regulatory mechanisms of PICK1 in PCa bone metastasis.

Main Methods:

  • Immunohistochemistry (IHC) to assess PICK1 expression in 198 PCa patients.
  • Statistical analysis correlating PICK1 with clinicopathological features.
  • In vitro (PC-3, C4-2B cells) and in vivo (mouse intracardial model) experiments to study PICK1 function.

Main Results:

  • Reduced PICK1 expression in metastatic PCa tissues and bone-derived cells.
  • Downregulation of PICK1 correlates with higher PSA, Gleason grade, and bone metastasis.
  • PICK1 overexpression suppresses PCa cell invasion, migration, and bone metastasis.
  • miR-210-3p overexpression causes PICK1 downregulation in metastatic PCa.
  • Inverse correlation between PICK1 and miR-210-3p in PCa tissues.

Conclusions:

  • PICK1 downregulation, driven by miR-210-3p, is a key mechanism in PCa bone metastasis.
  • This uncovers a novel epigenetic regulatory pathway for TGF-β signaling in PCa bone metastasis.

More Related Videos

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells06:54

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

14.6K
Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model06:32

Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model

Published on: November 4, 2022

7.0K

Related Concept Videos

TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.7K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.7K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.9K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.8K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.4K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
10.8K