Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ICP1 bacteriophage treatment antagonizes colonization of the zebrafish larval intestine by <i>Vibrio cholerae</i>.

Microbiology spectrum·2026
Same author

PIKfyve mediates the maturation of mycobacteria-containing vesicles.

Frontiers in cellular and infection microbiology·2026
Same author

Protective versus deleterious roles of pyroptosis in Mycobacterium avium and Mycobacterium marinum infections.

Cell death & disease·2026
Same author

Interaction of HS1BP3 with cortactin modulates TKS5 localisation, cell secretion and cancer malignancy.

Molecular oncology·2026
Same author

The RAB27A effector SYTL5 regulates mitophagy and mitochondrial metabolism.

eLife·2025
Same author

Pneumolysin-dependent and independent non-canonical autophagy processes mediate host defense against pneumococcal infection.

Autophagy·2025
Same journal

Correction: Verde et al. Molecular Mechanisms of Protein Aggregation in ALS-FTD: Focus on TDP-43 and Cellular Protective Responses. <i>Cells</i> 2025, <i>14</i>, 680.

Cells·2026
Same journal

Inflammation in Cardiomyopathies: Cellular Mechanisms Across Cardiac Phenotype.

Cells·2026
Same journal

IL-4/IL-13-Driven Dysregulation of Epidermal Lipid Metabolism in Atopic Dermatitis: An Immunometabolic Link Between Type 2 Inflammation and Barrier Dysfunction.

Cells·2026
Same journal

Activity of DNA- and RNA-Guided Prokaryotic Argonautes in Human Mitochondria.

Cells·2026
Same journal

Placental Pathophysiology in Maternal Psychoactive Substance Use: Biological, Clinical, and Forensic Perspectives.

Cells·2026
Same journal

PACAP and Maxadilan (PAC1 Agonist) Influence Plaque Progression, Migratory Ability, and Mitochondrial Morphology and Dynamics in Vascular Smooth Muscle Cells.

Cells·2026
See all related articles

Related Experiment Video

Updated: Feb 26, 2026

Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions
12:52

Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions

Published on: September 9, 2014

16.5K

Studying Autophagy in Zebrafish.

Benan John Mathai1, Annemarie H Meijer2, Anne Simonsen3

  • 1Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Sognsvannsveien 9, 0317 Oslo, Norway. b.j.mathai@medisin.uio.no.

Cells
|July 13, 2017
PubMed
Summary
This summary is machine-generated.

Autophagy, a cellular recycling process, is vital for health and disease prevention. This study reviews methods for analyzing autophagy in zebrafish, a promising new model organism.

Keywords:
GFP-Lc3aggrephagyautophagyconfocal microscopymitophagyxenophagyzebrafish

More Related Videos

High-fat Feeding Paradigm for Larval Zebrafish: Feeding, Live Imaging, and Quantification of Food Intake
11:30

High-fat Feeding Paradigm for Larval Zebrafish: Feeding, Live Imaging, and Quantification of Food Intake

Published on: October 27, 2016

11.1K
Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration
08:14

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

Published on: May 20, 2015

9.2K

Related Experiment Videos

Last Updated: Feb 26, 2026

Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions
12:52

Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions

Published on: September 9, 2014

16.5K
High-fat Feeding Paradigm for Larval Zebrafish: Feeding, Live Imaging, and Quantification of Food Intake
11:30

High-fat Feeding Paradigm for Larval Zebrafish: Feeding, Live Imaging, and Quantification of Food Intake

Published on: October 27, 2016

11.1K
Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration
08:14

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

Published on: May 20, 2015

9.2K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Autophagy is a fundamental cellular process responsible for degrading damaged components and maintaining homeostasis.
  • Dysfunctional autophagy is implicated in aging and various diseases, including neurodegenerative disorders and cancer.
  • Understanding autophagy mechanisms is crucial for developing therapeutic strategies.

Purpose of the Study:

  • To provide an overview of methods for analyzing autophagy in zebrafish.
  • To highlight the utility of zebrafish as a model organism for autophagy research.
  • To discuss current findings and future directions in zebrafish autophagy studies.

Main Methods:

  • Review of established and emerging techniques for autophagy analysis in zebrafish.
  • Discussion of genetic and chemical approaches to modulate autophagy.
  • Description of imaging and biochemical assays for monitoring autophagic flux.

Main Results:

  • Zebrafish offer a powerful platform for studying autophagy due to their optical transparency and genetic tractability.
  • Various methods, including fluorescent reporters and biochemical assays, are effective for analyzing autophagy in zebrafish.
  • Current research has identified key roles for autophagy in zebrafish development and disease models.

Conclusions:

  • Zebrafish are a valuable model for dissecting autophagy pathways and their roles in health and disease.
  • Further research in zebrafish holds potential for uncovering novel therapeutic targets for autophagy-related disorders.
  • Standardized methods and continued investigation will advance the field of autophagy research using zebrafish.