Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antimicrobial Efficacy of Endogenous Blue Light Photoinactivation (400-470 nm) Against <i>Escherichia coli</i>: A Systematic Review of In Vitro Evidence and Clinical Implications.

Medical sciences (Basel, Switzerland)·2026
Same author

Chemokine-Receptor Modulation Shapes Neuroinflammatory and Biomechanical Responses in Organotypic Hippocampal Cultures After Oxygen-Glucose Deprivation.

ACS chemical neuroscience·2026
Same author

Validation of contact mechanics models for atomic force microscopy <i>via</i> finite elements analysis and nanoindentation experiments.

Nanoscale·2026
Same author

Correction to "Near-Equilibrium Unbinding of Streptavidin-Biotin Using Single Molecule Acoustic Force Spectroscopy".

Nano letters·2026
Same author

Indenting multicellular spheroids with various tip geometries.

European biophysics journal : EBJ·2026
Same author

Lipid Saturation and Cholesterol Drive the Mechanical Response of Lipid Bilayer to Ionic Liquid: An Atomic Force Microscopy Study.

Langmuir : the ACS journal of surfaces and colloids·2026

Related Experiment Video

Updated: Jun 20, 2026

Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy
11:10

Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy

Published on: August 28, 2011

Standardized Nanomechanical Atomic Force Microscopy Procedure (SNAP) for Measuring Soft and Biological Samples.

Hermann Schillers1, Carmela Rianna2, Jens Schäpe2

  • 1Institute of Physiology II, University of Münster, 48149, Münster, Germany.

Scientific Reports
|July 13, 2017
PubMed
Summary
This summary is machine-generated.

A new standardized nanomechanical AFM procedure (SNAP) enables reliable measurement of soft sample elasticity, including living cells. This method significantly reduces variability in measurements, improving cell mechanics applications.

More Related Videos

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
14:13

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid
08:58

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid

Published on: December 2, 2022

Related Experiment Videos

Last Updated: Jun 20, 2026

Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy
11:10

Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy

Published on: August 28, 2011

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
14:13

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid
08:58

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid

Published on: December 2, 2022

Area of Science:

  • Biophysics
  • Materials Science
  • Cell Biology

Background:

  • Atomic Force Microscopy (AFM) is crucial for nanomechanical characterization.
  • Accurate determination of elastic modulus in soft samples, like living cells, is challenging due to measurement variability.
  • Existing AFM force spectroscopy methods require precise optical lever system adjustment for reliable data.

Purpose of the Study:

  • To develop a standardized procedure for reliable elastic modulus determination using AFM.
  • To minimize instrument, laboratory, and operator-dependent errors in AFM measurements.
  • To validate the procedure's effectiveness across different labs and sample types.

Main Methods:

  • Introduced the Standardized Nanomechanical AFM Procedure (SNAP).
  • Ensured precise AFM optical lever system adjustment.
  • Calculated deflection sensitivity using vibrometer-determined spring constants to eliminate errors.

Main Results:

  • Reduced variability in hydrogel elastic moduli measurements to 1%.
  • Increased consistency in living cells elasticity measurements by a factor of two.
  • Validated the procedure across a network of European laboratories.

Conclusions:

  • SNAP provides highly reproducible elasticity measurements for soft samples and living cells.
  • Improved consistency enhances the applicability of cell mechanics as a quantitative diagnostic marker.
  • The procedure ensures reliable, instrument-independent nanomechanical measurements.