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Gamma-glutamyltranspeptidase in murine lymphomas
European Journal of Cancer & Clinical Oncology
|January 1, 1986
Summary
Murine lymphoma cell lines show low gamma-glutamyltranspeptidase (GGT) activity. This enzyme
Area of Science:
- Biochemistry
- Oncology
- Immunology
Background:
- Gamma-glutamyltranspeptidase (GGT) is an enzyme with various physiological roles.
- Its activity in different cell types, including tumors, is of research interest.
Purpose of the Study:
- To quantify gamma-glutamyltranspeptidase (GGT) content and activity in murine lymphoma cell lines.
- To investigate GGT levels in both in vitro and in vivo tumor models.
Main Methods:
- Assay of GGT content and specific activity in five murine lymphoma cell lines.
- In vivo studies using a highly malignant S49 lymphoma variant in BALB/c mice.
- Histochemical examination of tumor infiltrations in host tissues.
Main Results:
- All tested lymphoma cell lines exhibited detectable but low GGT specific activities in vitro.
- In vivo tumors (solid and ascitic) derived from the S49 line showed consistently low GGT levels.
- Tumor cell infiltrations in liver and spleen were GGT-negative; liver showed elevated host GGT.
Conclusions:
- Low gamma-glutamyltranspeptidase (GGT) levels are characteristic of tumors originating from the immune system.
- GGT activity in lymphoma models is generally low and not significantly affected by tumor type or growth conditions.