Related Experiment Video
Updated: Feb 26, 2026
09:30
Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
Published on: January 18, 2017
12.6K
Towards sensitive, high-throughput, biomolecular assays based on fluorescence lifetime
Anastasia Ioanna Skilitsi1, Timothé Turko2, Damien Cianfarani1
1Université de Strasbourg, CNRS, Institut de Physique et Chimie des Matériaux de Strasbourg, UMR 7504, F-67000 Strasbourg, France.
Methods and Applications in Fluorescence
|July 13, 2017
Summary
This study introduces time-correlated single photon counting with droplet microfluidics for sensitive biomolecular interaction sensing. This robust, cost-effective method achieves high-throughput detection for enzymatic assays.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Microfluidics
Background:
- Biomolecular interaction sensing is crucial for diagnostics and drug discovery.
- Existing methods often face limitations in throughput, sensitivity, or cost.
- Time-resolved fluorescence offers enhanced specificity for molecular detection.
Purpose of the Study:
- To develop a robust and sensitive method for detecting biomolecular interactions.
- To implement time-correlated single photon counting (TCSPC) in high-throughput droplet microfluidics.
- To demonstrate the cost-effectiveness and sensitivity of this integrated technology.
Main Methods:
- Utilizing droplet microfluidics for rapid, low-volume sample handling.
- Implementing time-correlated single photon counting (TCSPC) for fluorescence lifetime detection.
- Developing an enzymatic activity assay using fluorescently-labeled biomolecules.
Main Results:
- Achieved highly sensitive detection of biomolecular interactions.
- Demonstrated reliable discrimination between positive and negative control samples.
- Reached a high throughput of several hundred samples per second.
Conclusions:
- Time-resolved fluorescence detection using TCSPC in droplet microfluidics is a sensitive and cost-effective technology.
- This approach enables robust and high-throughput sensing of biomolecular interactions.
- The developed method shows significant potential for various biochemical assays.
Related Concept Videos
01:19Protein Dynamics in Living Cells
2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K
01:14Photoluminescence: Applications
1.2K
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
1.2K