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Updated: Feb 26, 2026
Fluorescence Angiography for Evaluation of Aneurysm Perfusion and Parent Artery Patency in Rat and Rabbit Aneurysm Models
Published on: July 24, 2019
Fluorescent test accurately predicts leak of ischemic colon anastomosis in rats
Laura Moschetti1, Tiago Leal Ghezzi2, Belisa Gomes Müller3
1MSc, Postgraduate Program in Surgical Sciences, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre-RS, Brazil. Conception and design of the study; surgical procedures; acquisition, data, analysis and interpretation of data; statistical analysis, manuscript preparation and writing; critical revision; final approval.
Purpose::
To investigate if fluorescein fluorescent test can predict dehiscence in a model of ischemic colonic anastomosis in rats.
Methods::
This experimental controlled trial randomly assigned 55 rats to four groups. Anastomoses were performed in non-ischemic colon segments (control group) and in ischemic colon segments measuring 1, 2 or 3 cm long (groups 1, 2 and 3, respectively). Fluorescein was injected and the tissues were examined under ultraviolet light. Seven days later, a second-look surgery was performed to check for the presence or absence of anastomosis dehiscence.
Results::
Twenty-four rats presented anastomotic dehiscence during the second-look surgery. Reticular and nonfluorescent patterns were significantly associated with the occurrence of anastomotic dehiscence. Fluorescein fluorescence had a sensitivity of 95.8%, specificity of 89.2%, positive predictive value of 88.4%, negative predictive value of 96.2%, and accuracy of 92.3% to predict anastomotic dehiscence.
Conclusion::
Fluorescein fluorescent test can accurately predict leak in a model of ischemic colonic anastomosis in rats.
