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In vivo full-field functional optical hemocytometer.

Fuli Zhang1,2, Mingyi Wang1, Dingan Han1

  • 1Department of Photoelectric Technology, Foshan University, Guangdong, China.

Journal of Biophotonics
|July 13, 2017
PubMed
Summary

We developed a lab-free optical hemocytometer for live specimens, utilizing an absorption intensity fluctuation modulation effect to count red blood cells in capillaries. This technology provides real-time flow video, velocity, and red blood cell counts for circulation studies.

Keywords:
absorptionbiomedical imagingfull-fieldhemocytometerin vivointensity fluctuation modulationmicrocirculation

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Area of Science:

  • Biomedical Optics
  • Physiology
  • Microcirculation

Background:

  • Accurate red blood cell (RBC) counting is crucial for understanding blood circulation.
  • Existing methods often require complex laboratory setups or invasive procedures.
  • Non-invasive, real-time monitoring of RBC dynamics in live specimens is needed.

Purpose of the Study:

  • To introduce a lab-free, in vivo full-field functional optical hemocytometer (FFOH).
  • To leverage the absorption intensity fluctuation modulation (AIFM) effect for enhanced RBC detection.
  • To enable simultaneous measurement of flow video, velocity, and RBC count in capillaries.

Main Methods:

  • Utilizing low-coherence light illumination to detect absorption differences between RBCs and background tissue.
  • Implementing the AIFM effect to generate endogenous intensity fluctuations as RBCs flow through capillaries.
  • Computing real-time modulation depth to distinguish and quantify RBC signals.
  • Developing a full-field optical hemocytometer for simultaneous video, velocity, and count acquisition.

Main Results:

  • Demonstrated successful in vivo application of FFOH in live biological specimens.
  • Achieved real-time, label-free detection and quantification of RBCs in capillary networks.
  • Simultaneously captured flow video, measured flow velocity, and determined RBC count.
  • Validated the efficacy of the AIFM effect for highlighting RBC signals against background tissue.

Conclusions:

  • The FFOH system offers a novel, non-invasive approach for studying blood circulation.
  • This technology has potential applications in understanding physiological mechanisms in near-transparent live samples.
  • FFOH provides a valuable tool for real-time microcirculation research without the need for laboratory processing.