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Following Cell-fate in E. coli After Infection by Phage Lambda

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Bacteriophage D: an IncD group plasmid-specific phage.

J N Coetzee, D E Bradley, G Lecatsas

Journal of General Microbiology

|December 1, 1985

Summary

Researchers confirmed plasmid incompatibility group D using compatibility experiments and isolated a specific phage, phage D. This phage targets IncD plasmid-encoded pili, aiding in the characterization of this plasmid group.

Area of Science:

Microbiology
Molecular Biology
Virology

Background:

Plasmid incompatibility groups are crucial for understanding plasmid maintenance and host range.
Plasmid incompatibility group D (IncD) was previously tentatively identified.

Purpose of the Study:

To reaffirm the existence of the IncD plasmid group.
To further delineate the IncD plasmid group through phage isolation and characterization.

Main Methods:

Compatibility experiments were performed on plasmids R687, R711b, R778b, and R840.
A specific phage, designated phage D, was isolated.
Phage adsorption and plaque formation assays were conducted on various bacterial strains carrying IncD plasmids.
Phage morphology and nucleic acid content were analyzed.

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Main Results:

Compatibility experiments confirmed the IncD plasmid group.
Phage D specifically adsorbed to pili encoded by IncD plasmids in Escherichia coli, Salmonella typhimurium, Proteus morganii, and Klebsiella oxytoca.
Plaque formation was temperature-sensitive (26°C but not 37°C) and varied in morphology across different hosts.
Phage D did not propagate on IncD plasmid-carrying strains of Providencia alcalifaciens, Providencia stuartii, or Serratia marcescens.
Phage D is an RNA-containing phage with an isometric hexagonal outline, sensitive to chloroform, and adsorbs to IncD plasmid-coded pili.

Conclusions:

The existence and delineation of the IncD plasmid incompatibility group were confirmed.
Phage D serves as a specific marker for IncD plasmids and provides insights into their encoded pili structure and function.